Session: SAT 164-196-Pituitary
Poster Board SAT-175
Hypothesis: Craniopharyngiomas are a developmental disease in which aberrations in the normal epigenetic regulatory events may lead to the development of craniopharyngioma instead of the normal pituitary gland. In addition, craniopharyngiomas with a propensity to recur or progress have different miRNA expression profiles from those that are less aggressive.
Methods: Primary and recurrent formalin-fixed, paraffin-embedded (FFPE) craniopharyngioma samples from nineteen patients, ten age-matched pituitary samples from autopsy, and ten infant pituitary samples from autopsy were obtained from the Department of Pathology at Ann and Robert H. Lurie Children’s Hospital of Chicago. Total RNA was extracted and miRNA expression evaluated using the Applied Biosystems TaqMan® Real-Time PCR with over 700 miRNAs represented. RealTime StatMiner® (Integromics®) was used to identify the differentially expressed miRNAs between tumors and controls as well as tumors that recurred compared to those that did not recur.
Results: Approximately thirty differentially expressed miRNAs were identified in craniopharyngiomas when compared to infant pituitary samples and when compared to age-matched controls (p ≤ 1x10-8). Many of the miRNAs identified were down-regulated in both of the comparisons, while other differentially expressed miRNAs were unique when compared to either the age-matched controls or infant pituitary samples. Additionally, miR-199a-5p was identified to be down-regulated in craniopharyngiomas that recurred as opposed to those which did not have a recurrence (p=0.05).
Conclusions: There are multiple miRNAs that are differentially expressed in craniopharyngiomas as compared to both normal and infant pituitary tissue. These miRNAs may play a role in the development and biological behavior of craniopharyngiomas. Additionally, miR-199a-5p may play a role in craniopharyngiomas that recur and may function as a prognostic marker or potential therapeutic target.
Nothing to Disclose: JHS, EFV, FFC, RLH, STS, SG, TT, DZ, MBS
*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm
See more of: Abstracts - Orals, Featured Poster Presentations, and Posters