AROMATASE EXPRESSION CORRELATES WITH TGF-Β1 EXPRESSION IN HUMAN TESTICULAR PATHOLOGIES

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SUN 524-553-Male Reproductive Endocrinology
Bench to Bedside
Sunday, June 16, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SUN-525
Candela Rocio Gonzalez1, Oscar Levalle2, Claudio Terradas3, Elisa Puigdomenech3, Roberto Ponzio4, Alfredo Vitullo1, Calandra Saul Ricardo*5 and Silvia Ines Gonzalez-Calvar4
1Research Center of Biomedical Biotechnology, Environmental and Diagnostic Studies, Buenos Aires, Argentina, 2Durand Hospital, Buenos Aires, Argentina, 3Medical Institute PREFER, Buenos Aires, Argentina, 4School of Medicine, Buenos Aires, Argentina, 5Institute of Biology and Experimental Medicine, Buenos Aires, Argentina
In certain pathologies of male reproduction, such as Sertoli Cells Only Syndrome (SCO) or Idiopathic Hypospermatogenesis (H), a marked testicular interstitial proliferation is observed. We recently described in patients with SCO and H showing Leydig cell hyperplasia (LCH) a greater expression of TGF-β1 and its co receptor endoglin, suggesting the involvement of this cytokine in the proliferative process (Reprod Biol Endocrinol 8:148-159, 2010). The aim of the present work was to analyze, the expression of PCNA, aromatase and estrogen receptors (ERα/ERβ) in testicular biopsies of patients with SCO (n = 6), H (n = 6) and SCO and H also presenting HCL (n = 4). Furthermore, we studied the influence of TGF-β1 on aromatase and ERα/ERβ gene expression in the Leydig cell line TM3 by real time PCR. Studies by immunohistochemical techniques indicate the presence of aromatase in Leydig cells and increased expression of PCNA in the group HCL. Also, the co-localization of ERα/β were detected in all pathologies by immunofluorescence. An increase in gene expression of aromatase in biopsies with HCL respect to H and SCO were observed by RT-qPCR (SCO = 0.76 ± 0.38; SCO + HCl = 3256.99±318.25; H = 1.58 ± 0.67, H + HCL = 4196.45± 1806.94). In all patients studied, levels of aromatase expression correlated positively with the levels of TGF-β1 evaluated in previous experiments (r2 = 0.7628, Person’s Coefficient: 0.8734, p<0.0001).On the other hand, the “in vitro” stimulation of TM3 Leydig cells with TGF-β1 (1 ng/ml) induced an increase in gene expression of aromatase (10 fold). Furthermore, ERα/β were significantly induced by TGF-β1 (1 ng / ml) (p<0.05).

In conclusion, in patients with SCO or H associated with HCL, the increased in aromatase gene expression and its correlation with the levels of TGF-β1 indicate that these factors might be crucial in the mechanism leading to the proliferation of Leydig cells.

Nothing to Disclose: CRG, OL, CT, EP, RP, AV, CSR, SIG

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm

Sources of Research Support: Supported by CONICET (PIP2011:#150) and Felipe Fiorellino Foundation- Maimónides University.