Session: FP11-Pediatric Endocrinology
Room 104 (Moscone Center)
Poster Board SAT-596
Objective and Method: We used Cox proportional hazard models to investigate whether promoter DNA methylation of CYP19A1 and PPARG, independently or in concert with body mass index (BMI), was associated with age at pubic hair development (PH2) or breast development (B2) in a cohort of 6-8 year-old Black and Hispanic New York City girls (n=125) who were between 6-8 years-old at enrollment. Percent methylation of specific CpG dinucleotides in these promoters was quantified in buccal DNA by bisulfite pyrosequencing. Overweight was defined as BMI greater than or equal to the 85t h percentile for age.
Results: An inverse association between CYP19A1 and PH2 was suggested (HR = 0.95, CI= 0.90-1.00, p = 0.05). CYP19A1 methylation was not independently associated with breast development, but appeared to modify timing of B2 when BMI was considered (BMI-CYP19A1 interaction p = 0.085). Specifically, compared to normal weight girls with ‘high’ CYP191A1 methylation (reference), overweight girls with ‘low’ CYP191A1 methylation had a higher risk of early B2 (HR = 2.15; 95% CI= 1.23- 3.76). Similar trends were suggested for PH2 when CYP19A1 and BMI were considered together, but were not statistically significant. No significant associations between PPARG and pubertal development were detected.
Conclusion: Our findings suggest the DNA methylation status of a specific CYP19A1 promoter may influence timing of breast development in overweight girls. Consistent with other emerging reports, these observations indicate epigenetic biomarkers of risk may be identified in non-invasively collected surrogate tissues.
Nothing to Disclose: TRS, MSW, ST, AP, JC
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