Session: MON 389-405-Signaling Originating from Membrane Receptors
Poster Board MON-396
Methods: Sprague-Dawley adult female rat bladders were examined by immunohistochemistry (n=12) and molecular forms of neurotrophins examined by Western blotting (n=12) using bladder extracts prepared at physiological pH in the presence of detergents and protease inhibitors.
Results: The βNGF antibody immunostained structures in suburothelial mucosa while BDNF and NT3 antibodies immunostained the urothelial cells. The NT-4 antibody immunostained the neural plexuses in suburothelial regions. The βNGF antibody immunostained nerve fibers innervating the detrusor smooth muscle layers while the BDNF and NT3 antibodies immunostained the detrusor smooth muscle cells. The NT4 antibody immunostained the neural plexus localized between muscle layers. βNGF antibody identified a 75, 35, 25 and 13kDa proteins. The BDNF antibody identified a 150 and a 15kDa protein. The NT-3 antibody identified a 250 and 18kDa protein and the NT-4/5 antibody identified a 25kDa protein.
Summary: All four neurotrophin antibodies distinctly immunostained the urothelial and muscle layers in the rat bladder. Our finding identified for the first time the high molecular weight neurotrophin precursor molecules in this organ. The molecular sizes of the neurotrophin precursors are in close agreement with studies reported in the innervated peripheral organs, central nervous system and cultured immune cells.
Conclusion: We postulate that neurotrophin precursors are likely to play a key role in bladder functions. Any aberration in proneurotrophin expression, processing and function could contribute to the pathophysiology of painful bladder syndrome and other bladder dysfunctions particularly in the elderly.
Nothing to Disclose: JL, EMR, MH, FSW, MGF, RM
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