Session: SAT 326-337-Hormone-Dependent Tumors
Bench to Bedside
Poster Board SAT-337
Methods: We collected serum and plasma samples in 61 healthy subjects. All samples were analyzed in a single laboratory using CGA ELISA assays from CisBio (both serum and plasma), Alpco (both serum and plasma), Dako (plasma only), the RIA from CisBio (plasma only) and a new flash chemiluminescent method from Invitron (plasma only). Reference ranges were determined using a bootstrap non-parametric procedure, and Passing-Bablok non-parametric regression. Paired T tests were used to compare results across assays and plasma vs. serum values respectively.
Results: The reference ranges were calculated for CGA values specified by assay and medium. There was considerable variation across assays both for plasma and for serum, with serum ranges often significantly lower then plasma ranges. Further, for several assays, the reference range that we established was significantly different from the reference range provided in package inserts.
Conclusions: Reference ranges for CGA in healthy subjects may considerably differ from package insert references. Reference ranges need to be established separately for serum and plasma. This information is critical for correct decision-making in the care of patients with NEN’s.
Nothing to Disclose: KKC, HHM, AD, WK, LA, SV
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