The expression of tight junction genes in murine placenta

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 498-514-Female Reproductive Endocrinology
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-506
Changhwan Ahn and Eui-Bae Jeung*
College of Veterinary Med, Cheongju Chungbuk, South Korea
Tight junctions (TJs) are composed of a branching network of sealing strands. TJs regulate paracellular conductance and ionic selectivity. TJ components include the peripheral protein ZO-1, junctional adhesion molecules (JAMs) and the integral proteins such as occludin and claudin. claudins are a family of proteins that are the most important components in the tight junctions. They establish paracellular transport barriers that control transportation of molecules within intercellular space. The present study focused on the expression of claudin, suggesting as major working molecules in the paracellular transport system. To study the mechanisms and roles of claudin family, the expression levels of claudin family in various organs should be provided. In this article, we examined expression of mouse placental claudin family. Pregnant C57/BL6 mice were used in this study and tight junction proteins including Claudin-1 to Claudin-24, JAM-a, Zo-1, and occludin, tricellulin, MarvelD3 expressions. In the transcription levels, Claudin-1, Claudin-4, Claudin-6 expression levels were relatively high compared to other claudin family. Especially Claudin-4 protein was predominantly expressed than other tight junction proteins. Claudin-4 has been known as a responsive gene for a direct decrease in paracellular conductance by selectively reducing the permeability of Na+ ions. On the other hand, Claudin-8, Claudin-9, Claudin-13, Claudin-14, Claudin-15, and Claudin-18 were expressed weakly, Claudin-10a and Claudin-16 were not detectable by Real time PCR in our experimental condition. Other claudin proteins were expressed moderately. Since the transcriptional levels of tight junction genes were expressed variously, the protein levels and their localization in the placenta will be further evaluated by immunostaining and Western blot assay. This study will provide the data of the tight junction gene expressions and their localization in the mouse placenta, which may contribute to assuming the roles of these tight junction genes regarding the maternal-fetal ion transportation in the placenta.

Nothing to Disclose: CA, EBJ

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