Fas gene expression in human adipose tissue is related to obesity, insulin resistance and type 2 diabetes

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 842-862-Insulin Signaling & Action
Basic/Translational
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-851
Matthias Blueher1, Nora Klöting1, Stephan Wueest2, Eugen J Schoenle2, Michael R Schön3, Arne Dietrich1, Mathias Fasshauer1, Michael Stumvoll1 and Daniel Konrad*2
1University of Leipzig, Leipzig, Germany, 2Univ Children's Hosp, Zurich, Switzerland, 3Städtisches Klinikum Karlsruhe, Karlsruhe, Germany
Objective – Deletion of the death receptor Fas (CD95) in adipocytes of mice is associated with improved insulin sensitivity and reduced adipose tissue (AT) inflammation. Here we investigate the role of AT Fas expression in human obesity.

Research Design and Methods – In paired samples of omental and subcutaneous (SC) AT from 256 lean and obese participants, we investigated whether Fas mRNA expression is fat depot-specific, altered in obesity and related to measures of AT inflammation and insulin sensitivity.

ResultsFas is significantly higher expressed in omental compared to SC AT. Fas expression correlates with BMI (OM, r²=0.44, SC, r²=0.14), AT macrophage infiltration (OM, r²=0.36, SC, r²=0.16) and glucose infusion rate in euglycemic-hyperinsulinemic clamps (OM, r²=0.17, SC, r²=0.13) (p<0.05 for all). Insulin sensitive had significantly lower Fas expression than insulin resistant obese individuals.

Conclusions –Independently of body weight, increased Fas expression may contribute to impaired insulin sensitivity and AT dysfunction in obesity.

Nothing to Disclose: MB, NK, SW, EJS, MRS, AD, MF, MS, DK

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm