Session: SAT 649-659-Basic Mechanisms of Obesity
Poster Board SAT-655
Objectives: The aim of this study was to characterize the consequences of GHD on adiposity, total body weight (TBW), and food intake in a mouse model of autosomal recessive isolated GHD due to targeted ablation of the GH-releasing hormone (GHRH) gene [GHRH knockout (GHRHKO)]. Animals were also analyzed with respect to leptin, adiponectin and visfatin gene expression in both intra-abdominal and subcutaneous fat.
Design: We studied 8 male mice homozygous for GHRHKO allele (-/-) and 8 male heterozygous animals (+/-) as controls. Feeding and TBW data were collected weekly beginning 3 months through 5 months of age, after which animals were sacrificed. Body length (nose-anus distance, N-A), intra-abdominal (epididymal and retroperitoneal) and subcutaneous (inguinal) fat weights were measured. Total RNA was extracted from both intra-abdominal and subcutaneous fat and reverse transcribed to evaluate gene expression of leptin, adiponectin and visfatin by real-time reverse transcription polymerase chain reaction (real-time RT PCR). Data were statistically analyzed by unpaired t test.
Results: Compared to controls, GHRHKO mice, despite decreased TBW and N-A (P<0.0001), had significantly increased relative intra-abdominal (P<0.01) and subcutaneous (P<0.0001) fat, accompanied by significantly increased food intake per TBW (P<0.01) and unmodified TBW change. Adiponectin and visfatin mRNA levels were significantly decreased in both intra-abdominal (P<0.001) and subcutaneous fat (P<0.0001) of -/- mice. No significant difference in leptin mRNA levels was detected.
Conclusions: GHD due to targeted ablation of the GHRH gene in mice is associated with increased relative intra-abdominal and subcutaneous fat mass, a significant increase in food intake, and a decrease in adiponectin and visfatin gene expression in both fat depots.
Nothing to Disclose: LB, RS, LR, CF, SL, AC, CD, AR, FM, GO, RS, MV
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