INCREASED GENOM INSTABILITY AND OXIDATIVE DNA DAMAGE IN ACTIVE ACROMEGALIC PATIENTS

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 167-198-Hypothalamus-Pituitary Development & Biology
Basic/Clinical
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-183
Fahri Bayram*1, Nazmiye Bitgen1, Hamiyet Donmez-Altuntas1, Ilkay Cakir2, Zuhal Hamurcu1, Ali Kurtsoy1, Fatma Sahin1, Yasin Simsek1, Halit Diri1, Züleyha Cihan Karaca1 and Gulden Baskol1
1Faculty of Medicine, Erciyes University, Kayseri, Turkey, 2Numune Hospital,SIVAS, SİVAS, Turkey
Acromegaly is a chronic condition resulting from the excessive secretion of growth hormone (GH) and insulin-like growth factor 1 (IGF1), which is mitogenic and has a more fundamental role in oncogenic transformation and development of mammary cell carcinoma. High concentrations of circulating IGF-1 have been associated with increased cancer risk in humans in several epidemiological studies. The objective of this study is to assess both chromosomal DNA damage, cytototoxicity, cytostasis by using cytokinesis-block micronucleus cytome (CBMN cyt) assay and oxidative DNA damage by using 8-hydroxy-2'-deoxyguanosine (8-OHdG) assay in patients with active acromegaly and controls. CBMN cyt assay is a method used to measure DNA damage (micronucleus: MN; nucleoplasmic bridges: NPBs; and nuclear buds: NBUDs), cytototoxicity (proportions of apoptotic and necrotic cells) and cytostasis (proportions of mono-, bi- and multinucleated cells; nuclear division index: NDI). The study was carried out in 30 patients who had active acromegaly and in 30 age- and sex-matched healthy controls. MN frequency, the number of NPBs and NBUDs in lymphocytes of patients with acromegaly were found to be significantly higher than that in controls (p<0.001, p<0.001, p<0.001, respectively). The frequency of apoptotic and necrotic cells in lymphocytes of patients with acromegaly were found to be significantly higher than that in controls (p<0.001 and p<0.001 respectively). No statistically significant differences in the number of metaphase, the numbers of 2 nucleated cells (M2), 3 nucleated cells (M3),  4 nucleated cells (M4) and NDI values  were observed in between patients and control groups (p>0.05). Also, the correlation between increased MN frequency and elevated serum IGF-1 levels in acromegalic patients was statistically significant. Plasma 8-OHdG (ng/ml) levels by 2-fold in patients with acromegaly were found to be significantly higher than that in controls (p<0.005). In addition to elevated serum IGF-1 levels in acromegalic patients, both elevated chromosomal DNA and oxidative DNA damage may predict an increased risk of malignancy in this patients. It is of vital importance that patients with acromegaly be followed up for cancer risk.

Nothing to Disclose: FB, NB, HD, IC, ZH, AK, FS, YS, HD, ZCK, GB

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm