Session: SAT 381-386-Steroid Hormone Actions, Biosynthesis & Metabolism
Poster Board SAT-385
We found that the expression of LacZ was abnormally maintained in an abnormally retained fetal zone through P30 in the mutant male mouse. A similar retention of the fetal zone has been observed in mice and in patients with loss-of-function mutations in Dax1 (Nr0B1). This result is in contrast to the normal regression of the fetal zone at puberty in wildtype male mice. Because we have shown previously that FAdE-mediated expression of Sf1 is maintained by auto-activation of FAdE by Sf1 itself, we hypothesize that SUMOylation of Sf1 is important for full extinction of FAdE enhancer activity and ultimate regression of the fetal zone. Further in-vitro studies using FAdE-luciferase constructs confirm that SUMO-tagged Sf1 is markedly deficient in activating FAdE activity in 293T cells compared to SUMO-deficient Sf1. Future efforts will focus on examining the molecular mechanism by which SUMOylation of Sf1 together with Dax1 regulates FAdE activity during development.
Disclosure: GDH: Consultant, orphagen, Founder, Atterocor, Consultant, Embera, Advisory Group Member, Embera, Consultant, HRA Pharma, Consultant, Corcept, Consultant, Isis, Advisory Group Member, orphagen, Consultant, OSI-Astella. Nothing to Disclose: YX, MZ, HAI
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