Session: MON 515-547-Female Reproductive Endocrinology
Poster Board MON-521
Objective:To investigate the mechanism(s) responsible for the change in AC structure and function of higher primate females.
Hypothesis:Regulation of gonadotropins following ovariectomy (OVX) by different patterns of ovarian steroid leads to specifically different changes in the structure and function of the AC.
Experimental Design: Intact control and long-term OVX female nonhuman primates (n=24) were either intact and untreated (n=6) or OVX and treated with vehicle (n=6), estrogen alone (E2, n=6) or estrogen plus progesterone (E2+P, n=6) for 7 to 12 months. Animals ranged in age from 7-25 years. The adrenal glands from all animals were then evaluated for structural and functional changes.
Results:The structure and function of the AC were modulated differently by endogenous sex steroids and hormone replacement therapy (HT) regimens. Preliminary immunohistochemical staining of fixed sections revealed the presence of luteinizing hormone receptors (LHR) in all three AC layers, with staining differences reflecting age, ovarian status and HT.
Summary:These data provide direct evidence that the structure and function of the AC of higher primate females can be modulated by changes in ovarian status via steroid hormone secretion patterns. These results also demonstrate that HT regimens can simulate the action of the ovary on the AC during the MT and may be responsible for the changes that are observed in the secretion of adrenal steroids in most mid-aged women.
Conclusion: These findings suggest that elevated circulating luteinizing hormone (LH) levels through activation of their receptors in AC contribute to adrenal steroid secretion during MT. The response of the AC to different patterns of ovarian steroid levels associated with different regimens of HT provides a plausible mechanism to explain the rise in adrenal steroid production during and following the MT for most women.
Nothing to Disclose: BLL, CVR, ML, AJC, DMH, FFV, NAG, DRC, DSM, AJD, FZS, JHM
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