Session: FP22-Testis Biology
Room 104 (Moscone Center)
Poster Board SUN-532
We have previously shown that MCs are significantly increased in testes of infertile men (3). Now, by laser capture microdissection technique and PCR, we detected the expression of the mel1a receptor and antioxidant enzymes in testicular MCs.
Physiological studies cannot be performed on human testicular biopsies and human testicular MC lines are not available. Therefore, non-testicular human HMC-1 MCs which express mel1a receptors were used to further characterize the interactions between melatonin and the intracellular redox state.
Incubation of MCs in the presence of H202 (10-4 M) significantly induced the expression of catalase and SOD1 evaluated by real-time PCR and/or Western blot, and the generation of reactive oxygen species (ROS) determined by fluorescence. A 10-7M dose of melatonin further increased the expression of catalase, SOD1 and peroxiredoxin 1 and generated lower levels of ROS than H2O2 alone. In contrast, a 10-5M concentration of melatonin increased SOD1 expression but did not affect catalase and peroxiredoxin 1 expression. Unexpectedly, 10-5M melatonin generated higher levels of ROS than H2O2alone.
Taking together, the findings indicate that melatonin is a potential modulator of the testicular oxidative state by acting at least on the local MC population. Moreover, melatonin shows a dual effect on MC redox state being antioxidant at low levels but prooxidant at high concentrations.
Nothing to Disclose: SPR, SW, MEM, CT, RP, EP, OL, RSC, AM, MBF
*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm
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