Session: SAT 338-357-Steroid Hormone Actions
Poster Board SAT-347
Methods: 24 male rats were divided in 4 groups; intact, castrate, castrate+ low T, castrate+ medium T, castrate + high T and treated for 6 weeks. After treatment, animals were euthanized, serum and prostates were harvested. QRT-PCR was used to evaluate transcriptional levels of the genes that are involved in androgen/AR signaling pathways. LC-MS/MS was used to determine the levels of T and DHT in both serums and prostates.
Results: Rats in low T group only had 23% as much T in serum as the intact group while they had 63% as much DHT and 71% as much T in the prostate compared to the rats in the intact group, demonstrating the ability of healthy rat prostate tissue to maintain androgen levels despite low systemic levels. Low T treatment resulted in significant alterations in expression of androgen axis genes, while these changes were not as strong with mid and high T treatments.
Conclusions: Our study suggested that regardless of the serum level of T and DHT, the prostate could work as buffer system to maintain a functional level of T and DHT. Long exposure to low T could promote upregulation of genes that participate in steroidogenesis and induce overexpression of AR, suggesting that intracrine steroidogenesis as well as increased androgen responsiveness might contribute to the sustained levels of T and DHT in the prostate. However, long-term study is needed to investigate whether low systemic T has a causal relationship with the onset of PC and whether changes in the androgen/AR signaling axis in response to low circulating T may contribute to prostate tumorigenesis.
Nothing to Disclose: YZ, MO, MH, JJ
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