The relationship between vitamin D and inflammatory markers in older individuals

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SUN 257-280-Disorders of Vitamin D Metabolism & Action
Sunday, June 16, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SUN-269
Marcello Maggio*1, Fulvio Lauretani2, Antonio Cherubini3, Francesca De Vita4, Chiara Cattabiani5, Stefania Bandinelli6, Simonetta Morganti7, Graziano Ceresini8, Luigi Ferrucci9 and Gianpaolo Ceda1
1University of Parma, Parma, Italy, 2University-Hospital of Parma, 3Italian National Research Centres on Aging (INRCA), Cosenza Italy., 4University of Parma, Italy, 5University of Parma, Italy, Parma, Italy, 6Geriatric Unit, Azienda Sanitaria Firenze, Florence, Italy, 7University-Hospital, Parma, 8Univ of Parma, Parma, Italy, 9NIA/NIH, Baltimore, MD
During aging there is a progressive reduction in  vitamin D levels and an increase in inflammatory markers (1). A causal relationship has been hypothesized but never tested in a population study. Vitamin D could control inflammation through regulation of the nuclear proinflammatory transcription factor kappa B (NF-kB). 25-OH D-deficient subjects  have an increased expression of NF-κB and of the inflammatory cytokine Interleukin-6 (IL-6) (2). Therefore, we hypothesize an inverse and independent relationship between vitamin D levels and inflammatory markers.                                                                                                                                                                                                                                                                                           Methods.The study population  consisted of  1002  male and  female (382 men and 497 women)  65 yrs or older  residents in the area of Chianti, Tuscany, Italy.  223  participants  with  C-reactive protein (CRP)  higher than 10 mg/dl, on chronic treatment on corticosteroids and nonsteroidal anti-inflammatory drugs  were excluded, leaving  the final sample of 879 subjects  with complete data on serum concentrations of vitamin D, CRP, IL-6, and  soluble interleukin-6 receptor (sIL6r ). Serum 25(OH)D was measured by RIA (RIA kit; DiaSorin, Stillwater, MN). Intra- and interassay coefficients of variation were 8.1 and 10.2%, respectively. Serum IL-6, sIL-6r, were measured in duplicate by high-sensitivity ELISAs (BioSource International, Camarillo, CA). The lower detectable limit was 0.1 pg/ml for IL-6, 8.00 pg/ml for sIL-6r. The interassay coefficient of variation was 7% for all cytokines. CRP was measured with a high-sensitivity ELISA, a competitive immunoassay that uses purified protein and polyclonal anti-CRP antibodies. The interassay coefficient of variation was 5 %. The minimum detectable concentration was 0.03 mg/liter. Statistical analyses were  performed using  general  linear  models  adjusted for age, sex, BMI,  physical activity, chronic  diseases, and  parathyroid hormone (PTH).Vitamin D and inflammatory cytokines were log transformed because of skewed distribution.                                                                                                                                                                                                                        Results The mean age at baseline was 75.14 ± 17.16 years.  In the analysis adjusted  for age, sex and  PTH, vitamin D levels  were  significantly and  inversely  associated with IL-6   (β±SE -0.10±0.02, P<0.0001)  and  CRP  (β±SE -0.04±0.02, P=0.041)  and positively associated with sIL6r (β±SE 0.10±0.03, P=0.006). In the multivariate analysis adjusted for age, sex, BMI, caloric and alcohol intake, smoking, physical activity, cognitive  function, depressive status, Parkinson disease, peripheral artery disease, COPD, chronic heart failure, cancer, log (Vit D) was  negatively  associated  with  log (IL-6)  (β±SE -0.09±0.03, p<0.0001) and positively  associated  with  log (sIL6r) (β±SE -0.10±0.03, p<0.0001).                                                                                               Conclusion.Vitamin D levels were independently and inversely associated  with  IL-6  and  positively associated  with  sIL6r in older  population suggesting a potential anti-inflammatory  role for Vit D. Longitudinal studies are needed to better delineate the direction of this association.

1)      Lips P (2001) Vitamin D deficiency and secondary hyperparathyroidism  in the elderly: consequences for bone loss and fracturesand therapeutic implications. Endocr Rev 22:477–501 2)      Querfeld U. Vitamin D and inflammation. Pediatr Nephrol. 2012 Dec 13. 3)      Sohl E, de Jongh RT, Heijboer AC, Swart KM, Brouwer-Brolsma EM, Enneman AW, de Groot CP, van der Velde N, Dhonukshe-Rutten RA, Lips P, van Schoor NM. Vitamin D status is associated with physical performance: the results of three independent cohorts.   Osteoporos Int. 2013 Jan;24(1):187-96. doi: 10.1007/s00198-012-2124-5. Epub 2012 Sep 8.

Nothing to Disclose: MM, FL, AC, FD, CC, SB, SM, GC, LF, GC

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