Session: FP33-Insulin Signaling & Inflammation
Room 303 (Moscone Center)
Poster Board MON-846
Using whole-cell patch clamp recordings followed by single cell PCR, we find that insulin mRNA is strongly expressed in an identified subset of GABAergic interneurons in the cerebral cortex of the rat. These results were confirmed by immunocytochemistry detecting insulin in a subpopulation of GABAergic cells. Insulin mRNA copy numbers vary according to extracellular glucose concentrations as detected by single cell digital PCR in interneurons which were characterized electrophysiologically and reconstructed anatomically. In addition, gene chip analysis performed on these identified GABAergic cells comparing hyper- and hypoglycemic conditions suggests changes in the expression of several genes functionally linked to insulin. Moreover, our experiments show that insulin release enhancing compounds promote de novo insulin synthesis in brain slices as detected by radioimmunoassay. Furthermore, targeted electrophysiological application of the same compounds to interneurons expressing insulin mimics the effect of external insulin on local microcircuits via insulin receptors.
These results identify a subset of neocortical GABAergic interneurons as sources of insulin in the central nervous system and propose a link between GABAergic and insulin mediated action in cortical microcircuits.
Nothing to Disclose: GT, GM, NF, AKK, MR, SL, EB, RB, JG, LGP, EC
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