Session: SUN 366-382-Physiological Impacts of Endocrine Disrupting Chemicals
Poster Board SUN-370
The mechanism of ED action is most often explained by binding to nuclear steroid receptors. EDs can also act via nonnuclear steroid hormone receptors, nonsteroid receptors, orphan receptors and influence enzymatic pathways involved in steroid biosynthesis and/or metabolism.
Our research is focused on an additional possible mechanism of endocrine disruptor´s action. We hypothesized that EDs could affect the activity of 11β-hydroxysteroid dehydrogenase (11βHSD) type 2. The role of 11βHSD type 2 in testes represent the conversion of active stress hormone cortisol into inactive cortisone and thus protection of the target tissue from excess of glucocorticoids. Since glucocorticoids inhibit expression of lutropin receptors, an excess of cortisol in Leydig cells can lead to decrease of testosterone levels that are essential for sperm production.
To evaluate an impact of selected EDs on activity of 11βHSD we correlated cortisol/cortisone ratio from seminal fluid with the serum levels of polychlorinated biphenyls (PCBs, 4 classes) in men.
The preliminary study consists of 52 men (age 35.45 ± 5.54) divided into the groups according spermiogram values: healthy men (A, n=39), lightly infertile men (B, n=8), moderately infertile men (C, n=3), and severely infertile men (D, n=2). One-way ANOVA followed by multiple comparison test was used to compare groups. Statistical significance was found when comparing cortisol/cortisone ratio in groups (mean ratio A: 0.63; B: 1.16; C:0.78; D:0,37; p=0.0041). The ratio was the highest in lightly infertile men in comparison with other groups; it means their 11βHSD activity was the lowest. Subsequently, the activity of 11βHSD in moderately infertile men was lower than in healthy men and severely infertile men. There wasn´t significant difference among groups in serum PCBs levels (mean concentration-pg/g, A: 1,61; B: 1.85, C:1.92; D1,55; NS).
Our pilot study revealed that the activity of 11βHSD was lower in lightly as well as moderately infertile men compared with healthy men and severely infertile men but the association with PCBs serum levels was not observed.
This work was supported by grant IGA NT/13369-4 and IGA NT/12349-4 from the Czech Ministry of Health.
Nothing to Disclose: JK, JH, RH, MB, MH, LS, LS
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