Vitamin A affects the fat accumulation during bovine intramuscular adipogenesis

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SUN 649-677-Adipocyte Biology
Sunday, June 16, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SUN-663
Akira Hasegawa*, Daiki Taniguchi, Toshio Harigaya and Yasushi Mizoguchi
Meiji University, Kawasaki Kanagawa, Japan
Vitamin A (VA) is a very important nutrient, with important roles in body functions such as vision, growth promotion and protein composition. It is known that VA can affect lipid metabolism in ruminants and non-ruminants. Retinol (Ro) in animals has various derivatives, such as retinal (Ra), all-trans retinoic acid (ATRA) and 9-cis retinoic acid (9cRA), and can be produced from forms of provitamin A such as beta carotene (BC). In this study we investigated the effects of different forms of VA on the amount of lipids in a bovine intramuscular preadipocyte (BIP) cell line, which had been derived from the intramuscular adipose tissue of the musculus longissimus thoracis of Japanese Black cattle. We cultivated BIP cells for up to 12 days after adipogenetic stimulation that had been treated with 1 μM Ro, Ra, ATRA, 9cRA or BC, or was untreated. The BIP cells were harvested at 0, 3, 6, 9 and 12 days after adipogenetic stimulation. Lipids were extracted as triglyceride (TG), according to the Bligh and Dyer method, and the amount of TG was measured by the enzymatic method. 9cRA (days 6 & 9) increased the amount of TG after adipogenetic stimulation compared with the untreated cells (p = 0.001 and 0.004, respectively). And ATRA (all sampling days) tended to increase the amount of TG. There was no significant effect with either Ra or BC, but treatment with Ro resulted in significantly reduced TG on day 6 (p = 0.02). Therefore, these results show that different forms of VA altered fat accumulation. ATRA and 9cRA increased the amount of TG during adipogenesis. It is well known that ATRA and 9cRA derivatives of VA, which showed increased TG during adipogenesis, have retinoic acid receptors and retinoid X receptors; these receptors are strongly related to fat accumulation. Therefore, the results of our study suggest that bovine fat accumulation during adipogenesis can be altered by changing the form of VA. This study should help improve understanding on how fat accumulation is affected by VA.

Nothing to Disclose: AH, DT, TH, YM

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