FP05-2 Connection of the CNR1 Polymorphisms (rs806368, rs12720071, rs1049353, 806381, rs10485170, rs6454674, rs2023239) With Nonalcocholic Fatty Liver Disease in PCOS and Healthy Controls

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: FP05-Lipids: Regulation & Mechanism of Disease
Saturday, June 15, 2013: 11:00 AM-11:30 AM
Presentation Start Time: 11:05 AM
Room 133 (Moscone Center)

Poster Board SAT-724
Andrzej Jan Milewicz*1, Lukasz Laczmanski2, Justyna Kuliczkowska2, Agnieszka Lenarcik2, Katarzyna Kolackov2, Maurycy Pawlak2, Felicja Lwow3 and Marek Bolanowski4
1Medical University Wroclaw, Wroclaw, Poland, 2Wroclaw Medical University, Wroclaw, Poland, 3University School of Physical Education, Wroclaw, Poland, 4Wroclaw Univ Med School, Wroclaw, Poland
Nonalcocholic fatty liver disease (NFLD) may be evident in woman with PCOS, both conditioning associating with metabolic disorders. Endocannabinoids modulate eating behavior; hence, endocannabinoid genes may contribute to the biological vulnerability to eating disorders, obesity, fat cumulation and others. The aim of our study were to develop the hypothetical role of the CNR1 polymorphisms in etiology of nonalcocholic fatty liver disease in woman with PCOS (diagnosis on the ESHRE/ASRM criteria) in comparison to homogenous healthy control.

208 woman with PCOS and 120 woman as control were study. NFLD were diagnose on the basis of USG and biochemical markers. Genomic DNA were isolated using standard method. To polymorphisms identification PCR and minisequencing were used. Reaction products were separated on Genetic Analyser ABI 3100. For statistic analysis Hardy-Weinberg Equilibrium was used.

62% woman with PCOS presented NFLD in comparison to 48% in control. Our results showed significantly higher frequency of the A/A genotype of the rs806381 (p<0. 001258) and G/G genotype of the rs10485170 (p<0.000027) in woman with PCOS and NFLD versus PCOS woman without NFLD. These specific correlation was not observed in control group. We observed also significantly higher (p<0.001718) frequency of the A/A genotype of rs1049353 CNR1 polymorphism vs. controls. As well as G/G genotype of the rs806381 CNR1 polymorphism (p<0.000086) vs. controls. The relation between CNR1 polymorphisms to hormonal and metabolic profile will discuss.

Our preliminary results suggest potencial role of the CNR1 polymorphisms in etiology of the NFLD specially in PCOS woman what need further study.

(1) Baranova et al. Aliment. Pharmacol. Ther. 2011; 33: 801-814 (2) Carmina J. Hepatol. 2007; 47: 313-315 (3) Milewicz et al. Int J Obes 2011; 35: 373-377

Nothing to Disclose: AJM, LL, JK, AL, KK, MP, FL, MB

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm