Session: MON 842-862-Insulin Signaling & Action
Poster Board MON-855
RESEARCH DESIGN AND METHODS―In palmitate-treated HepG2 cells as well as high fat diet (HFD)-fed SD rats and db/db mice, hepatic SeP expression and its regulatory pathway, including AMPK-FOXO1α, were evaluated after the administration of salsalate and salicylate.
RESULTS―Palmitate treatment significantly increased SeP expression and induced IR, while knock-down of SeP by siRNA restored these changes in HepG2 cells. Palmitate-induced SeP expression was inhibited by both salsalate and salicylate in dose- and time-dependent manners, which was found to be mediated by AMPK-FOXO1α pathway. Moreover, adiponectin were also able to suppress palmitate-induced SeP through the activation of AMPK and improve IR. Salsalate and salicylate treatment improved glucose intolerance and IR, accompanied by reduced SeP mRNA and protein expression in HFD-fed rats and db/db mice, respectively.
CONCLUSION―Salsalate and adiponectin ameliorated palmitate-induced IR in hepatocytes via SeP inhibition through the AMPK-FOXO1α pathway.
Nothing to Disclose: HCH, TWJ, SYL, HYC, JHA, YJK, NHK, HJY, HYK, JAS, NHK, SGK, SHB, DSC, KMC
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