Human Beige Adipocyte is stimulated by EID1 from ADMSC cells

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SUN 678-689-Adipocyte Biology
Clinical
Sunday, June 16, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SUN-685
Fernando Lizcano*1 and Diana Vargas2
1Universidad de La Sabana, Chia Cundinamarca, Colombia, 2Universidad de La Sabana, Chia, Cundinamarca, Colombia
Increased lipid accumulation in the form of triglycerides in the white adipose tissue induces obesity and increase cardiovascular risk. However, white adipose cells may have greater caloric expenditure by stimulating the expression of factors specific to brown fat cell and reduce complications of obesity. Here we show the role of EID1 in the control of adipogenic process in mesenchymal cells derived from human adipose tissue (ADMSC). EID1 described as muscle differentiation inhibitor reduces the activation of the nuclear receptor PPARg by blocking p300 and PRIP coactivators without affecting the activity of SRC-1. In ADMSC cells derived from healthy women, we found that overexpress EID1 decreases lipid accumulation and additionally active as UCP1 protein, PGC1a and TFAM, engaged in caloric expenditure which is characteristic of brown-like cells (beige). To investigate the specific role of EID1 in adipose cell differentiation, EID1 expression was knocked down using siRNA and a reduced PG1a and TFAM was observed. EID1 also binds to and inhibits the pRb retinoblastoma protein that plays an important role in regulating adipose cell differentiation. In conclusion, EID1 reduces lipid accumulation of subcutaneous fat cells derived from human and could mediate the acquisition of a phenotype of adipose beige cells. Therefore EID1 action in adipogenesis might be a promising therapeutic target for the treatment of obesity.

Nothing to Disclose: FL, DV

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm