Session: SAT 708-722-Obesity: Response to Interventions
Poster Board SAT-715
In the hypothalamus 48h HFD triggered the following changes: i) amplified the decrease in clock expression between 6am and 6pm with no effect on per2 expression, ii) abolished the circadian rhythm of the orexigenic neuropeptides NPY (p<0.05) and AGRP (p<0.05), but had no effect on POMC expression, and iii) mitigated the circadian changes in IL-6 (p<0.05) and IL-1β (p<0.05) expression. In the prefrontal cortex, HFD abolished clock expression rhythmicity (p<0.05). In contrast, per2rhythmicity was affected in both prefrontal cortex (p<0.05) and visceral adipose tissue (p<0.05).
Our data show that HFD rapidly affects clock and/or per2 gene expression in diverse tissues. In the hypothalamus these changes concur with loss of circadian variation in orexigenic neuropeptide expression, which could be associated with the observed modification in the pattern of food intake. Interestingly, the HFD-induced loss of rhythmicity in IL-1β and IL-6 expression levels results in these mice having more, less or similar levels of these interleukins compared to controls depending on the time of day. Thus, any eventual synchronicity between zeitgeber-related genes, food intake and inflammation is disrupted by HFD. The eventual consequences of this de-synchronization in terms of energy metabolism regulation remain to be elucidated.
Nothing to Disclose: FH, PS, ND, ED, FD, JA, JAC, MR
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