Session: SUN 524-553-Male Reproductive Endocrinology
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Poster Board SUN-551
Pregnant Wistar-Imamichi rats were obtained from the Institute for Animal Reproduction, Ibaraki, Japan. All the rats were maintained under a 12:12 light-dark cycle and food and water were available ad libitum throughout the experiment. From postnatal day 0 (PD0) to PD27, male pups were administered orally with either 5 mg / kg / day flutamide or sesame oil (control). Male offspring of both groups were tested for sexual behavior and preference test 3 times (between 15 and 18 weeks old). At 19 weeks old, all rats were sacrificed to collect blood samples and dissect organ specimens given below for analyzing peripheral effects of flutamide: coagulating gland, seminal vesicle, prostate gland, penis, testes and epididymis. Penile length was measured and plasma levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone were determined.
Although all rats in both flutamide and control groups showed mount activity in sexual behavior test, flutamide treatments significantly decreased number of intromissions and ejaculations, and increased latencies of mount, intromission and ejaculation, compared to those of the controls. In contrast, no significant difference was seen in sexual preference tests between the two groups. In the flutamide group, penile lengths and weights were decreased compared to those of the controls, while no significant difference in other organ weights per body weights and in the hormonal levels were found between the groups.
In conclusion, Long-term exposure to flutamide during development suppressed sexual behavior and penile growth but did not affect sexual preference and hormone levels in male rats. Although the suppression of sexual behavior may be caused by hypoplasia of penis, it is still possible that such exposure affects brain function because of increased latencies of sexual behaviors.
Nothing to Disclose: MK, HU, YH, GW, KT, TH, HT, GS, YK, MK
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