Session: SUN 338-365-Metabolic & Stress Receptors in Energy Homeostasis
Poster Board SUN-345
We show (i) qPCR revealed no human transgene expression in any tissue of untreated animals. However, LRH-1 transcript and protein expression was observed in mammary epithelial cells of dox-treated animals following both short- (three weeks) and long- (three months) term dox treatment. To further our findings, (ii) we demonstrated an increase in Ki-67 immunoreactivity in luminal epithelial cells of dox treated animals, suggesting that LRH-1 induces mammary epithelial cell proliferation in vivo, as it does in vitro.
Finally, (iii) altered mammary gland morphogenesis was observed in dox-treated animals as demonstrated by a reduction in lateral bud number at both 3 week and 3 month time points. This prompted us to investigate the expression levels of transforming growth factor (TGF-β); a known factor that inhibits mammary gland morphogenesis. We revealed an induction of TGF-β immunoreactivity and transcript levels in dox-treated animals. We conclude that the effects of LRH-1 on ductal morphogenesis and branching are likely mediated via activation of TGFβ.
This study characterises a novel LRH-1 transgenic mouse which enables further exploration into the roles of LRH-1 in mammary development and provides a valuable model to further develop LRH-1 antagonist’s in-vivo.
Nothing to Disclose: KAL, CDC, ALC
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