Session: SAT 708-722-Obesity: Response to Interventions
Poster Board SAT-716
Methods & Materials: A-FABP knockout (KO) mice and their wild-type (WT) littermates were fed with either standard chow (STC) or HFHC diet. Basic parameters, glucose tolerance test, insulin tolerance test were monitored during the experimental period. Mice were sacrificed after diet induction for seven months. Plasma sera were collected for biochemical analysis. Hepatic lipid profiles were determined. Hematoxylin and eosin (H&E) staining, Sirius-red staining and Oil red O staining were performed to determine the morphology, fibrosis and lipid accumulation of the liver tissues.
Results: Hepatic expression of A-FABP was significantly elevated in HFHC diet-induced WT mice. Serum alanine transaminase level was significantly lower in the A-FABP KO mice, on HFHC diet, compared to their WT littermates, accompanied by a significant reduction in diet-induced hepatic triglyceride content. HFHC diet-induced macrophage infiltration, fibrosis and steatosis in the liver were also diminished in A-FABP KO mice. Moreover, the A-FABP KO mice displayed improved glucose tolerance and insulin sensitivity, compared to their WT littermates, despite a greater increase in body weight on HFHC diet.
Conclusion: Our data suggest that A-FABP deficiency protects against HFHC diet induced steatosis, inflammation and fibrosis in the liver, in keeping with a significant role of A-FABP in the development of NASH.
Nothing to Disclose: KSL, RLH, IPL, LS, MZ, AX
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