Session: MON 37-82-Pheochromocytoma & Paraganglioma
Poster Board MON-45
Objective: The aim of the study was to examine if genotype specific differences exist in the expression of Hexokinases 1, 2 and 3, GLUT-1 and -3, vascular endothelial growth factor (VEGF) and carbonic anhydrase 9 (CA-9).
Methods: 30 primary PGLs collected from patients with hereditary mutations in SDHB (n=2), SDHD (n=4), VHL (n=2), RET (n=5), NF1 (n=1), MAX (n=1) and sporadic patients (n=15) were investigated. Patients whose genotype was tested negative for SDHA/B/C/D/AF-2, RET and VHL were considered as sporadics. Paraffin embedded sections of these tumors were investigated using immunohistochemical methods with diaminobenzidine as read-out. The staining pattern was assessed by an experienced pathologist and a score was given to each sample after multiplication of percentage of positively stained area with staining intensity (scale:0-4 from 0-absent to 4-strong).
Results: Hexokinase 1 and 2 showed a granular staining pattern typical of mitochondria associated proteins while Hexokinase 3 and VEGF showed cytoplasmic staining. GLUT-1 and -3 also showed a predominantly cytoplasmic staining with around 5-20% of the cells showing membrane staining. Further, increased expression of Hexokinase 2 and VEGF were observed in SDH related tumors when compared to sporadic and RET, NF1 and MAX tumors. No evidence for the expression of CA-9 was observed in the PGLs examined except in one VHL tumor where a membrane staining was observed.
Conclusions: Results indicate an evidence for increased glycolysis and angiogenesis at molecular level in the SDH related tumors.
Nothing to Disclose: JU, TD, BK, EO, RW, ARMMH, AM, HPK, FCJS, HJT
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