Session: MON 796-817-Diabetes Genetics & Epidemiology
Poster Board MON-811
Objectives: To evaluate the association between the rs1990760 (G/A) polymorphism and T1DM or its clinical characteristics in a Southeast Brazilian population, and to analyze the IFIH1 gene expression in mononuclear cells from T1DM patients according to different genotypes of this polymorphism.
Methods: We analyzed 527 patients with T1DM and 517 nondiabetic subjects. The polymorphism was genotyped by Real-Time (RT) PCR using TaqMan MGB probes. IFIH1 gene expression in 26 samples of mononuclear cells was evaluated using RT-qPCR.
Results: Genotype frequencies of the rs1990760 polymorphism did not differ between T1DM patients and nondiabetic subjects (P=0.139) Moreover, the A allele frequency was similar between T1DM and nondiabetic samples (0.53 vs. 0.50; P=0.129). In T1DM patients, glycemic and lipid profile, body mass index, and age of T1DM onset did not differ significantly among different genotypes of this polymorphism. However, A allele carriers showed lower levels of both systolic (128.5 ± 18.9 vs. 119.7 ± 18.2 mm/Hg, P=0.019) and diastolic (82.4 ± 13.5 vs. 76.6 ± 10.6 mm/Hg, P=0.004) blood pressures as compared to G/G genotype patients, adjusting for covariates. The frequency of arterial hypertension (AH) was also increased in A allele carriers with T1DM, adjusting for covariates (P=0.019). IFIH1 gene expression in mononuclear cells did not differ among the three genotypes of the rs1990760 polymorphism. Interestingly, IFIH1 gene expression was higher in mononuclear cells from T1DM patients with AH as compared with T1DM patients without AH [median 6.7 (range 1.7-41.2) vs. 1.8 (1.3-73.3), respectively; P=0.036].
Conclusions: The present study showed no association between the rs1990760 polymorphism and T1DM; however, the A allele was associated with protection to AH in T1DM patients as well as with an increase IFIH1 gene expression.
Nothing to Disclose: APB, LDAB, FSD, NEL, LHC, DC
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