Session: MON 676-684-Central Regulation of Appetite & Feeding
Poster Board MON-681
Subjects and Methods: Eight men, age (mean ± SD) 21.5 ± 2.7 yr; BMI 22.4 ± 2.5 kg/m2were studied in a single-blind, placebo-controlled study during two separate overnight admissions on the Clinical Research Unit. They received either a 4-hr (2300h-0300h) infusion of hydrocortisone or saline. The hydrocortisone infusion rate (mg/kg·h ) was 0.3 for the initial 3 min, 0.24 for 9 min, and then 0.135 until the end of the infusion. Plasma acyl- and desacyl-ghrelin levels (using an in-house two-site sandwich assay) and cortisol levels (Immulite 2000) were measured every 10 min for 16 hrs (1700h-0900h). Ghrelin data were analyzed by way of linear mixed models with Bonferroni correction during the sampling period from 2300h to 0300h and 0300 to 0700h. Cortisol data were analyzed using a paired t-test.
Results: Acyl- and desacyl-ghrelin responses (pg/mL) during or after saline vs hydrocortisone infusion were not significantly different from zero. Mean ghrelin differences [upper, lower 95% CI] from 2300h-0300h were: acyl- [0.22 (-7.39, 7.83), p=1.0] and desacyl- [-3.36 (-17.66, 10.95), p=1.0]. From 0300h-0700h they were: acyl- [8.68 (1.07, 16.28), p=0.056] and desacyl-[8.75 (-5.56, 23.05), p=0.4]. Cortisol levels (ug/dL) (mean ± SE) were significantly higher during the hydrocortisone infusion compared to the saline infusion: 2300h-0300h [28.9 ± 0.6 vs 3.5 ± 0.58, p<0.01] and 0300h-0700h [19 ± 0.77 vs 8.4 ± 0.55, p<0.01].
Conclusion: Short-term increase in circulating cortisol levels by exogenous hydrocortisone infusion does not suppress circulating nocturnal acyl- or desacyl-ghrelin levels. Thus, it is unlikely that endogenous cortisol levels are responsible for the diurnal pattern of ghrelin secretion; it is more likely that this reflects circadian control.
Disclosure: MOT: Founder, Ammonett Pharma, Recipient Award, Novo Nordisk, Advisory Group Member, Pfizer, Inc., Advisory Group Member, Ipsen, Advisory Group Member, Chaisma. Nothing to Disclose: RMN, JL, SSP, JP, LSF, BDG
*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm
See more of: Abstracts - Orals, Featured Poster Presentations, and Posters