EFFECT OF ARTIFICIAL SWEETENERS ON BLOOD GLUCOSE, GLP-1, PYY, INSULIN AND C-PEPTIDE LEVELS IN PATIENTS WITH TYPE 2 DIABETES

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SAT 824-833-GI Regulatory Peptides
Bench to Bedside
Saturday, June 15, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SAT-831
Sule Temizkan*1, Oguzhan Deyneli2, Mutlu Gunes1, Mehmet Yasar1, Dilek Yazici1, Nese Imeryuz3, Goncagul Haklar4, Onder Sirikci4 and Dilek Yavuz1
1Div of Endocrinology, Marmara Univ Hastanesi, Istanbul, Turkey, 2Marmara Univ. Hospital, Istanbul, Turkey, 3Div of Gastroenterology, Marmara Univ Hastanesi, Istanbul, Turkey, 4Div of Biochemistry, Marmara Univ Hastanesi, Istanbul, Turkey
OBJECTIVE: The goal of this study was to determine the effect of artificial sweeteners (aspartame or sucralose), on blood glucose, insulin, c-peptid, glucagon like peptid-1 (GLP-1) and peptid YY (PYY) levels in type 2 diabetic patients during a 75 g oral glucose tolerance test (OGTT).

RESEARCH DESIGN AND METHODS: Eight newly diagnosed drug-naive type 2 diabetic patients (mean age 51,5 ± 9,2; F/M:4/4) underwent 75 g OGTT. During OGTT glucose, insulin, c-peptide, GLP-1 and PYY were measured at 15 minutes intervals for 120 minutes. The OGTTs were performed at three sessions on different days, where subjects were given 72 mg aspartame in water, 24 mg sucralose in water or only water 15 minutes before OGTT, in a randomized order.

RESULTS: Glucose excursions were nearly superimposable in the three settings but only in the sucralose condition, the glucose level at the 60th minute of OGTT was significantly lower than only water setting (p=0.01). GLP-1 peak at 30 th  minute of OGTT and area under the curve (AUC) were significantly higher in sucralose (AUC 12740±4315 pmol/L per 120 min) and aspartame (AUC 11153±5760 pmol/L per 120 min) settings compared to only water setting (AUC 6090±3846 pmol/L per 120 min) (p=0.02 and p=0.02, respectively). PYY AUC was significantly higher in sucralose setting (AUC 117±93 ng/ml per 120 min)  than  only water setting (AUC 72± 51 ng/ml per 120 min) (p=0.02) but there  was no statistical difference between aspartame and only water settings (p=0.09). Insulin AUC tended to be lower in sucralose setting than only water setting (p=0.04) but there  was no difference in insulin levels and Insulin AUC between aspartame and only water settings (p=0,33). C-peptide excursions were superimposable in the all three settings. There was no statistical difference between sucralose and aspartam UAC of glucose, insulin, c peptide, GLP-1 and PYY (p=0.48, p=0.26, p=0.78, p=0.21, p=0,40 respectively).

CONCLUSIONS: Artificial sweeteners seem to have no effect on blood glucose levels during OGTT with the exception of sucralose which decreases the peak 60th minute blood glucose levels. However, artificial sweeteners synergize with glucose to enhance GLP-1 release in newly diagnosed type 2 diabetic patients. This increase in GLP-1 secretion may be mediated via stimulation of sweet taste receptors on L cells by artificial sweetener.

Nothing to Disclose: ST, OD, MG, MY, DY, NI, GH, OS, DY

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm