CORRELATION OF HORMONAL PATTERN AND PLASMA ANTIOXDANTS IN MIDDLE AGE MALES WITH OSTEOPOROSIS

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SAT 224-247-Osteoporosis I
Clinical
Saturday, June 15, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SAT-236
Antonio Mancini*1, Sebastiano Raimondo1, Francesco Ciro Tamburrelli1, Chantal Di Segni1, Mariasara Persano1, Roberto Festa2, Andrea Silvestrini1, Elisabetta Meucci1, Luca Tiano2, Gian Paolo Littarru2 and Alfredo Pontecorvi3
1Catholic University of the Sacred Heart, Rome, Italy, 2Polytechnic University of the Marche, Ancona, Italy, 3Catholic University School of Medicine, Rome, Italy
Male idiopathic osteoporosis is underestimated, despite its clinical and social importance; the underlying biochemical mechanisms are still poorly understood. Previously, we demonstrated low plasma Total Antioxidant Capacity (TAC) in hypogonadal patients. The aim of this study was to investigate oxidative stress as risk factor for bone fracture and its relationships with endocrine milieu. We enrolled 31 males (36-72 ys), affected by back pain/spine fracture due to trivial trauma and 10 healthy controls (30-48 ys). TAC was determined by  colorimetric assay, using the system H2O2-metmyoglobin as source of radicals and a chromogen (ABTS); the latency time (LAG) in the accumulation of ABTS.+, spectroscopically detectable, is proportional to antioxidants concentration. Coenzyme Q10 (CoQ10), lipophilic antioxidant, was also determined, by electrochemical method, to assess it oxidized and reduced forms, in 16 out of 31 patients. The following parameters were evaluated: testosterone, estradiol, insulin, IGF-1, PRL, FT3, FT4, TSH, PTH, Vitamin D, osteocalcin, beta-cross laps. Statistical evaluation was performed using Mann-Whitney test. The prevalence of IGF-1 defects (52.8±15.3 ng/ml) was 5/31 (suggesting GH deficiency, GHD, confirmed by GHRH+arginine test). Hypogonadism (mean testosterone levels 2.03±0.46 ng/ml) was present in 4/31. The 22 patients left did not show alterations in the hormonal parameters studied. Despite LAG levels did not differ between patients and controls (72.7±8.5 vs 75.0±6.0 sec), 12 out of 31 patients had low LAG levels (between 50 and 60 sec) irrespective of hormonal milieu. Moreover, we found significantly lower Vitamin D levels in hypogonadal subjects, than in patients with GHD and patients with normal hormonal parameters (10.7±5.8 ng/ml vs 19.7±17.7 and 22.7±9.7 respectively). Again, despite lower levels of total CoQ10  in GHD patients vs hypogonadal and other patients (0.54±0.28 ug/ml, vs 0.71±0.25 and 0.82±0.30, respectively), a higher ratio ox/red  CoQ10  was observed in patients with normal hormone milieu (16.67±1.75 vs 11.00±1.87 in GHD), similar to that of hypogonadal subjects (16.50±2.65%), suggesting that oxidative stress per se can be present in such a condition, irrespective of hormone values. These preliminary data suggest a possible involvement of oxidative stress in unexplained fractures even if further investigations are needed to establish a possible correlation with anabolic hormones involved in bone metabolism.

Nothing to Disclose: AM, SR, FCT, CD, MP, RF, AS, EM, LT, GPL, AP

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