A High-fat Diet Affects the Changes in Depot-specific Gene Expression that Occur Upon Differentiation of Murine Adipose Precursor Cells

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SUN 649-677-Adipocyte Biology
Sunday, June 16, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SUN-657
Lucia Martinez de la Escalera*1, Xarubet Ruiz-Herrera1, Yazmin Macotela1 and Carmen Clapp2
1National University of Mexico (UNAM), Queretaro, Mexico, 2National University of Mexico (UNAM), Querétaro, Mexico
The metabolic syndrome is associated with hypertrophic obesity and an inability of adipocyte precursor cells (APCs) to differentiate into new adipocytes capable of adequately managing the excess fat. Visceral APCs have higher requirements for differentiation in vitro than those from subcutaneous depots, and excess visceral but not subcutaneous fat is strongly associated with metabolic disease. All adipose tissue depots are intrinsically different, and understanding their characteristics may help explain the varying risk they pose. Thus, we compared ten preferentially expressed genes between visceral and subcutaneous APCs from mice fed a high-fat (HFD) or control-chow diet (CD) before and after induction of in vitro differentiation. APCs were isolated from male mice after 8 weeks on either diet using MACS separation technology. Two days before 100% confluence (day -2), cells were incubated with APC commitment factor bone morphogenetic protein 4 (BMP4); on day 0 they were treated with differentiation cocktail (IBMX, dexamethasone, insulin and rosiglitazone), and on day 3 with insulin alone. Cells were collected before BMP4 was added (day -2) and after the differentiation protocol was completed (day 9). Gene expression was analyzed using qRT-PCR. Of the ten genes analyzed, five [haptoglobin (hp), angiotensinogen (agt), cadherin-9 (cdh-9), vascular endothelial growth factor-c (vegfc) and matrix metalloproteinase-3 (mmp-3)] showed a marked rise in expression that was differentially affected by the HFD in association with their depot origin. In subcutaneous APCs, the HFD attenuated the rise of hp and agt observed with a CD but up-regulated vegfc and mmp-3. The HFD caused no apparent  change in expression of agt in APCs from the visceral depot, but it down-regulated mmp-3 and attenuated the rise of hp and vegfc. Finally, in APCs from both depots, the HFD enhanced the expression of cdh-9 on day -2, blunting its upregulation at the end of the differentiation protocol. The marked depot-specific attenuation or over-expression of these genes as a response to HFD and adipogenic stimuli may indicate their participation in the metabolic changes of adipose tissue biology arising from obesity.

Nothing to Disclose: LM, XR, YM, CC

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm

Sources of Research Support: CONACyT 164423; FOMIX 174984 both awarded to YM