Inflammation score is associated with insulin resistance but not with insulin secretion in type 2 diabetes

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SUN 780-806-Determinants of Insulin Resistance & Associated Metabolic Disturbances
Basic/Translational
Sunday, June 16, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SUN-794
Giuseppe Daniele*, Teresa Vanessa Fiorentino, Rodolfo Guardado Mendoza, Francesco Andreozzi, Deidre Winnier, Christopher Jenkinson, Devjit Tripathy and Franco Folli
University of Texas Health Science Center, San Antonio, TX
Obesity and type 2 diabetes (T2D) are characterized by low-grade inflammation. Cytokines are related with metabolic abnormalities that characterize obesity and T2D and their plasma levels are increased in both conditions. The aim of this study is to evaluate the relation between an integrated cytokine score and glucose metabolism of obesity and T2D. In a cross-sectional study, 20 type 2 diabetic patients (33.5±6.0 Kg/m2, A1c: 7.5±1.3%), already treated with metformin and/or sulfonylurea, and 18 control subjects (BMI: 32.2±6.2 Kg/m2, A1c: 5.5±0.3%) were studied. All subjects received: OGTT, euglycemic hyperinsulinemic clamp and measurement of fasting plasma osteopontin (OPN), TNF-α, IL-6, MCP-1 and fractalkine (FRK). Plasma cytokines values were stratified in quintiles to create cut-off points used to assign a score ranging from 0 assigned to lowest quintile to 4 assigned to highest quintiles. Total inflammatory score for each patient was calculated by sum of each cytokine score. Cytokines levels were higher in diabetic subjects as compared to non diabetic subjects (OPN: 6.1±1.2 vs. 3.1±0.7 ng/ml, TNF-α: 14.1±2.2 vs. 9.0±0.7 pg/ml, P=0.02; IL-6: 3.7±0.5 vs. 2.9±0.1 pg/ml, p=0.02; MCP-1: 420.4±42.4 vs. 224.6±23.5 pg/ml, P<0.001, FRK: 44.8±8.3 vs. 34.2±13.4, P=0.04). Total inflammatory score was higher in T2D patients as compared to non diabetic subjects (12.4±4.1 vs. 7.7±3.1; p=0.001, respectively) and was strongly inversely correlated with M value (r= -0.45; p=0.007), positively correlated with fasting plasma glucose (r=0.61; p<0.001), 1st and 2nd hour OGTT (r= 0.47 and r=0.55; p<0.001, respectively). Total inflammatory score was weakly correlated with insulin secretion and disposition index (r=0.19 and r=-0.31; p=0.06, respectively). This study demonstrated that an increased cytokine integrated score is associated with insulin resistance but not with insulin secretion. The inflammatory score could improve the prediction of metabolic abnormalities in patients with T2D

Nothing to Disclose: GD, TVF, RG, FA, DW, CJ, DT, FF

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