Hypoglycemic effects of crude extract of Cnidocolus chayamansa in Streptozotocin-Induced Diabetic Rats

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 758-775-Beta Cells, Glucose Control & Complications
Basic/Translational
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-771
Elda Leonor Pacheco-Pantoja*, Francis Yuliett Echeverria-Bobadilla, Victor Raul Lopez-Rivas, Amalia Palacio-Hernandez and Pedro Aquino-Hernández
Universidad Anahuac Mayab, Merida, Mexico
Being diabetes one of the main health problems in the world (1), the use of extracts manufactured from plants becomes an alternate treatment and pertinent research should be done on this field (2). Cnidoscolus chayamansa, is an endemic plant in the Southeastern of Mexico known for its easy and cheap growing requirements. C. chayamansa contains dihydromyricetin a flavonoid that is believed exerts hypoglycemic effects (3) . In the present study we obtained a hydro-ethanolic extract from the leaves of C. chayamansa (EEC) optimized and standardized through fractionation. Diabetes was induced in male Wistar rats (150-200g) using a single intraperitoneal injection of streptozotocin (80mg/Kg). We aimed to compare the standard oral treatment metformin (MET) against the EEC. The experiment design utilized diabetic and non-diabetic group. The diabetic animals were treated with EEC (n=5), metformin (MET) (n=5) or water (n=3). The non-diabetic group was treated with MET (n=3) or EC (n=3). The results indicated that after 4 weeks of treatment the blood glucose levels decreased in the diabetic group treated either with metformina or EEC about 50% showing no significant difference between EEC and MET. However, in the MET group we found that there was a higher incidence of blindness (n=4), whereas in the EEC diabetic group only one animal developed the condition.

Also, we analyzed the correlation between glucose change and weight gain change. In the diabetic groups treated with MET or EEC it was observed a significant correlation (p=0.003 and p=0.032 respectively). The control group did not show any significant correlation between weight gain change and glucose change (p=0.19). When we compared the MET and EEC diabetic groups correlation coefficients using the Fisher transformation, we found that the effects in both groups were not different (p=0.76).

In conclusion, the EEC showed similar effects in decreasing the blood glucose when compared to those animals treated with MET. The advantage of using an herbal extract lies in the fact that MET has undesirable side effects (5), in contrast to the EEC which apparently conferred a higher quality of life to treated animals, since less animal suffered from blindness. These observations are enticing and prospective work will include the investigation on the gene expression related to glucose metabolism when the EEC is administered (6).

(1) Smyth, S et al., Nat Med 2006; 12:75. (2) Rosado C et al., Rev Endocri y Nutr 2001; . 9:122–125.  (3) Oyagbemi AA et al; Afr J Med Med Sci 2010 39; Suppl:171.(4) Gonzalez M et al., Plant Food Hum Nutr 2003; 3:1. (5) Van L et al., J Clin Pharm Ther 2011; 36:376. (6) Adaramoye OA et al., Alcohol Alcohol 2011 46:451.

Nothing to Disclose: ELP, FYE, VRL, AP, PA

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