Insulin resistance regulation through resistin induced by a putative peptide hormone derived from bone

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SUN 780-806-Determinants of Insulin Resistance & Associated Metabolic Disturbances
Basic/Translational
Sunday, June 16, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SUN-801
Xiaoyong Guo, Jiewen Zhou, Jian Li, Dan Li and Pei-Gen Ren*
Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China
Insulin resistance regulation through resistin induced by a putative peptide hormone derived from bone

Xiaoyong Guo, Jiewen Zhou, Jian Li, Dan Li, Pei-Gen Ren

Objectives: Insulin resistance is one of the major syndromes of type 2 diabetes mellitus, which is a physiological condition that peripheral cells failed to respond to normal level of circulating insulin. Many factors were reported which can regulate this condition, including adipokines like adiponectin and resistin. In rodent, resistin was mainly produced by adipose tissue, whileas, which was secreted from macrophages in human. By using bioinformatic analysis, in vitro cell system, we found a putative peptide hormone derived from bone tissue, another currently emerged energy metabolism player, can significantly induce the expression of resistin in mature adipocytes. In this study, we will investigate the relationship between the new peptide regulator and the adipokine resistin, meanwhile, between bone and energy metabolism.

Methods: Bioinformatic analysis was used to predict potential functional peptides. A 15 amino acid peptide was synthesized and tested in in vitro cell system. Differentiated or non-differentiated adipocyte cell line 3T3-L1 cells were used for functional detection. Real-time qPCR method was used to investigate the change of adipokines expression in the cells.

Results: In the differentiated 3T3-L1 cells after 10 days treated in differentiating medium, adipogenesis makers LPL and aP2 were found significantly increased. Oil red staining also demonstrated the cells were differentiated with lipid accumulated inside cells. Compared to the non-differentiated 3T3-L1 cells, differentiated 3T3-L1 cells had an around 10 times higher expression of resistin. Compared to the non-peptide treated mature adipocytes, the cells treated with the new peptide hormone expressed around 4 times more of resistin.

Conclusions: The complex relationships among energy metabolism, adipose tissue and bone need to be interpreted to understand better of T2DM, metabolism syndrome, and bone remodeling. In this system, there are many peptide/protein factors involved in the regulations through variable pathways. But, there’re still many unexplained phenomena which may be controlled by unknown factors. In this study, we found a putative peptide hormone which can increase resistin expression. Understanding the relationship between resistin and the peptide, would help to reveal the mechanisms of energy metabolism and T2DM.

Nothing to Disclose: XG, JZ, JL, DL, PGR

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm

Sources of Research Support: Acknowlegements: This work was supported by the following grants. (1) The National Basic Research Program of China (2012CB526708); (2) Shenzhen Innovative Pharmacology and Biotherapy Pre-clinical Test Public Service Platform