Session: SAT 806-823-Gestational Diabetes
Poster Board SAT-823
RESEARCH DESIGN and METHODS:
Study Population: Five pregnant women with Type 2 DM/gestational DM treated with insulin/ glyburide who delivered at term and their offspring. Six normoglycemic pregnant women with normal pregnancy and delivery at term and their offspring served as controls. Mean HgbA1c in DM mothers: 5.8 % (± 0.6).
Cell Culture: HUVEC were isolated from fresh umbilical cords by collagenase digestion and grown in culture. Cells in passage 3-4 were used for all experiments.
IGF-1R Abundance by Flow Cytometry (FC): Cells were grown to 70-80% confluence in T25 cm2 flasks. HUVECs from DM offspring were maintained in higher glucose concentration (180mg/dL) to mimic intrauterine conditions. Cells were then exposed to media containing normal glucose (100mg/dL) for 72h. HUVEC from control offspring were grown in media with 100mg/dL glucose and then exposed to glucose concentration of 360mg/dL for 72h. Mannitol was used as osmolarity control.
IGF-1R Abundance by Western Blot (WB):HUVECs were cultured under the various glucose exposures as described above in 6 well plates. After overnight serum starvation, cells were lysed and WB analysis was carried out for IGF1R abundance.
RESULTS: No significant differences were seen in the IGF1R abundance in cells derived from offspring of diabetic or control groups. The mean ranks of diabetic and controls groups were 6.6 and 5.5 respectively; U= 18, Z = - 0.46, p= 0.32, r = 0.1 (by Mann–Whitney Utest). Also, there was no influence of exposure to hyperglycemia in vitro.
CONCLUSIONS: IGF1R abundance in HUVECs is normal in well-controlled diabetes. In addition, hyperglycemia does not influence IGF1R abundance, indicating other mechanisms are involved in altering function of the downstream signaling.
Nothing to Disclose: AM, AMT, SDC
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