Session: MON 112-141-Hypothalamus-Pituitary Development & Biology
Poster Board MON-118
Immunohistochemistry, in addition to a fluorescence observation, was performed for embryonic (E) pituitaries on day E15.5, E18.5, E19.5 and E20.5 using antibodies to SOX2 (stem/progenitor marker and expressing in the pituitary stem/progenitor cell), PRX1 (paired-related homeobox protein; mesenchymal and novel pituitary transcription factor expressing in the pituitary stem/progenitor cell) and GFP. We observed GFP-positive cells at an Atwell's recess of the anterior lobe on E15.5 as invading cells from surrounding area and at a vicinity of the blood vessels on E18.5. In the intermediate lobe, GFP-positive cells were firstly observed on E20.5. Immunohistochemistry for PRX1 showed the PRX1-positive cells were present in the pituitary cells, surrounding mesenchymal cells and invading cells at the Atwell's recess on E15.5 and E18.5. Double staining for SOX2 showed two populations in the GFP/PRX1-double positive cells with the positivity. In the intermediate lobe on E20.5, GFP-positive cells were expressed SOX2 and/or PRX1.
The present study demonstrated that GFP-positive cells, mostly FS cells, were certainly present in the embryonic anterior lobe. In consider the absence of SOX2, some of the GFP-positive cells are mesenchymal origin invading through the Atwell's recess at early period, followed by involving in pituitary vasculogenesis. In conclusion, FS cells are composing of two different origins and already participate in development of the embryonic pituitary.
Nothing to Disclose: HY, MH, SY, KH, KF, NK, MC, HU, MT, TK, TY, YK
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