Modulator effects of the fiber intake (nopal tortilla and wheat integral bread) on glucose metabolism in presence of the variants rs7903146 and rs12255372 of TCF7L2 gene in type 2 diabetic patients

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 796-817-Diabetes Genetics & Epidemiology
Translational
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-816
Ma. Monserrat López-Ortiz*1, Elva L Perez-Luque2, Ma Eugenia Garay-Sevilla3 and Elizabeth Tejero4
1Universidad de Guanajuato, Leon, Mexico, 2Univ de Guanajuato, Leon Guanajuato, Mexico, 3Univ de Guanajuato, Leon GTO, Mexico, 4Instituto de Medicina Genomica, Mexico, Mexico
Introduction: The fiber intake helps to have a better metabolic control in type 2 diabetic patients. Genetic variants inTCF7L2 gene show strong association with type 2 Diabetes Mellitus (T2DM). Some persons with a specific genotype have better benefits in response to fiber intake.

Objective: To investigate the effect of fiber intake (nopal tortilla and wheat integral bread) in presence of rs7903146 and rs12255372 of TCF7L2 gene on the glucose metabolism in type 2 Diabetes Mellitus 

Material and methods: The type 2 DM patients received an individualized isocaloric diet with contribution of 25% fiber of daily requirement. Diet 1: 74 patients consumed 6 gr of nopal tortilla fiber for 8 week, after include a washout period for 8 week. Diet 2; 63 patients consumed 5.64 gr of wheat integral bread fiber for 8 week. Anthropometric, metabolic, and hormonal Basal and final data were measured. Before to begin the diet, the patients were typified for the assignment to: group A (wild type) or group B (mutate). We used descriptive Statistics, t Student test.

Results: The insulin, GLP-1 and HOMA-IR levels are increased after diet 1, but in diet 2 only GLP-1 levels are decreased without consider the genotypes. In diet 1, the carriers of wild genotypes rs7903146 and rs12255372, showed significant changes on anthropometric markers, and increased of insulin, GLP-1 and HOMA-IR levels. In diet 2, there were decreased in anthropometric markers in both polymorphisms.  In the rs7903146CC genotype, triglycerides, HbA1c, insulin, and β-cell function decreased.  Significant decrease in glucose (p=0.009), and LDL-Cholesterol (p=0.05) levels in patients with rs7903146CT/TT genotype were observed. In carrier patients of rs12255372GG genotype, total cholesterol (p=0.04) and HbA1c (p=0.0007) decreased, and in carriers of rs12255372GT/TT genotype, diminished total cholesterol (p=0.005), LDL-cholesterol (p=0.02), and GLP-1 (p=0.03) levels.

Conclusions: In the patients with diet based in nopal tortilla, we observe an interaction with wild genotypes (rs7903146CC y rs12255372GG) with increase of insulin, GLP-1, and HOMA-IR. In diet based in wheat integral bread, the carrier patients of rs7903146CC genotype we observed interaction with decrease of insulin and β-cell function. We found a better response in of rs7903146CT/TT on glucose and LDL-cholesterol levels. However, in carrier patients of rs12255372GT/TT genotype the LDL-cholesterol and GLP-1 levels decreased.

This work was supported from Universidad de Guanajuato 2011 grant.

Nothing to Disclose: MML, ELP, MEG, ET

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm