Electrical acupuncture stimulation is superior to manual stimulation in enhancing insulin sensitivity in female rats with and without polycystic ovary syndrome

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SAT 532-553-Hyperandrogenic Disorders
Basic/Clinical
Saturday, June 15, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SAT-536
Milana Kokosar*1, Anna Benrick1, Julia Johansson2, Manuel Maliqueo1, Miao Sun3, Louise Mannerås Holm1 and Elisabet Stener-Victorin4
1Institute of Neuroscience and Physiology, Göteborg, Sweden, 2Institute of Neuroscience and Physiology, Gothenburg, Sweden, 3First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, China, 4University og Gothenburg, Goteborg, Sweden
Polycystic ovary syndrome (PCOS) is a common endocrine disorder causing reproductive and metabolic disturbances including ovulatory dysfunction, hyperandrogenemia, obesity and hyperinsulinemia, which often precede the development of type-2 diabetes. Acupuncture with low-frequency electrical stimulation, improves insulin sensitivity, lipid profile and modulates skeletal muscle and adipose tissue gene expression. We have shown that electrical and manual stimulation of acupuncture needles for 4-5 weeks induce different effects on insulin sensitivity but this warrants further investigation.

The objective was to demonstrate the acute effect of acupuncture with low-frequency electrical stimulation versus manual stimulation on insulin sensitivity. We also determined gene expression of key regulatory molecules in skeletal muscle and adipose tissue as well as tissue specific glucose uptake using a tracer technique.

Twenty-one-day old female rats received a dihydrotestosterone (DHT)-releasing pellet to induce PCOS (n= 47) while controls (n= 33) did not receive a pellet. At 14 weeks of age, control and PCOS rats were each randomly divided into three experimental groups; no-stimulation, manual stimulation and electrical stimulation. Manual groups were stimulated by rotating the needles every 5-minute during 45 minutes while electrical stimulation groups were given 2 Hz stimulation with an intensity evoking muscle twitches. Insulin sensitivity was measured by euglycemic-hyperinsulinemic clamp and glucose infusion rate was monitored at steady-state, during treatment and a 45-minute follow up period. A 2-deoxy-glucose tracer was given to a subset of animals and tissue specific glucose uptake was calculated. At the end of the clamp skeletal muscle, adipose tissue and ovaries were dissected for analysis of gene and protein expression.

Electrical acupuncture stimulation was superior to manual stimulation in enhancing insulin sensitivity in both control and PCOS rats. Interestingly, the effect of manual acupuncture was more pronounced in insulin resistant PCOS rats compared with control rats during stimulation. Rate of disappearance and 3H-2DG tracer uptake in PCOS rats was lower in adipose tissue and in the ovary than in controls, and were not affected by treatment. In soleus muscle, the mRNA expression of Adipor2, Adrb1 and Mapk1 was higher in PCOS rats than in controls and the gene expression was decreased after electrical stimulation. Whether changes in gene expression in soleus muscle after stimulation are reflected by changes in protein expression is ongoing.

Nothing to Disclose: MK, AB, JJ, MM, MS, LM, ES

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm

Sources of Research Support: This study was financed by grants from the Swedish Research Council (Project No. K2012-55X-15276-08-3), The Jane and Dan Olsson Foundations, The Novo Nordisk Foundation, The Hjalmar Svensson Foundation, The Adlerbert Research Foundation, Wilhelm and Martina Lundgrens’s Science Fund, the Swedish federal government under the LUA/ALF agreement (ALFGBG-136481).