Session: SAT 532-553-Hyperandrogenic Disorders
Poster Board SAT-536
The objective was to demonstrate the acute effect of acupuncture with low-frequency electrical stimulation versus manual stimulation on insulin sensitivity. We also determined gene expression of key regulatory molecules in skeletal muscle and adipose tissue as well as tissue specific glucose uptake using a tracer technique.
Twenty-one-day old female rats received a dihydrotestosterone (DHT)-releasing pellet to induce PCOS (n= 47) while controls (n= 33) did not receive a pellet. At 14 weeks of age, control and PCOS rats were each randomly divided into three experimental groups; no-stimulation, manual stimulation and electrical stimulation. Manual groups were stimulated by rotating the needles every 5-minute during 45 minutes while electrical stimulation groups were given 2 Hz stimulation with an intensity evoking muscle twitches. Insulin sensitivity was measured by euglycemic-hyperinsulinemic clamp and glucose infusion rate was monitored at steady-state, during treatment and a 45-minute follow up period. A 2-deoxy-glucose tracer was given to a subset of animals and tissue specific glucose uptake was calculated. At the end of the clamp skeletal muscle, adipose tissue and ovaries were dissected for analysis of gene and protein expression.
Electrical acupuncture stimulation was superior to manual stimulation in enhancing insulin sensitivity in both control and PCOS rats. Interestingly, the effect of manual acupuncture was more pronounced in insulin resistant PCOS rats compared with control rats during stimulation. Rate of disappearance and 3H-2DG tracer uptake in PCOS rats was lower in adipose tissue and in the ovary than in controls, and were not affected by treatment. In soleus muscle, the mRNA expression of Adipor2, Adrb1 and Mapk1 was higher in PCOS rats than in controls and the gene expression was decreased after electrical stimulation. Whether changes in gene expression in soleus muscle after stimulation are reflected by changes in protein expression is ongoing.
Nothing to Disclose: MK, AB, JJ, MM, MS, LM, ES
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