Session: MON 37-82-Pheochromocytoma & Paraganglioma
Poster Board MON-47
After sample dilution with internal standard, deproteinisation is carried out using 10 K centrifugal filters. 75 µL of deproteinised sample is loaded onto a Waters Acquity OSM system, using weak cation exchange cartridges for further on-line sample clean-up. Chromatography is carried out on a Waters Hilic 3 µm 2.1x 50 mm column, and mass spectrometry is performed on a Waters Xevo TQS mass spectrometer. 3-MT and metanephrine are separated chromatographically in this assay.
The recovery of samples from the centrifugal filters was >95% for all 3 analytes at a concentration of 3 nmol/L. The LLOQ was 0.0375 nmol/L for metanephrine, and 0.075 nmol/L for normetanephrine and 3-MT. The assay was linear up to 30 nmol/L for all analytes, and a good correlation was shown between this assay and the assay currently in use in our laboratory for metanephrine and normetanephrine, with an r2 value >0.99.
We have developed a sensitive assay for plasma metanephrine analysis, which correlates well with our current assay for metanephrine and normetanephrine. The LLOQ of 3MT is 0.075 nmol/L, which will enable us to distinguish between normal and raised levels, something which has not been possible with previous assays.
Nothing to Disclose: BGK, JA
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