Session: FP09-Obesity: Physiologic Responses to Energy Balance Disruption
Bench to Bedside
Room 307 (Moscone Center)
Poster Board SAT-677
Methods Male Sprague-Dawley rats subjected to a novel paradigm, consisting of short-term exposure to recurrent restraint stress and antidepressants for 2 weeks, followed by long-term high-fat diet intake, were studied for 295 days. Body and organs weights, and behaviour were characterised.
Results: During the post-stress recovery period, obesity prone rats treated with fluoxetine had increased body weight (R-FX) in comparison to the control group treated with saline (R-C) and non-restraint control group (NRCF) (R-FX vs R-C: 610.6 ±15.52vs503.4±10.75g, P <0.0001; R-FX vs NRCF: 610.6 ±15.52vs566.1±14.55g, P<0.05). Fluoxetine-treated animals also had heavier bones in comparison to control groups (R-C vs R-FX: 1.995±0.06vs2.39±0.07g, P<0.0001; NRCF vs R-FX: 1.996±0.03vs2.39±0.06g, P<0.0001). Furthermore, spleen weight in saline treated animals was significantly decreased, while antidepressant treated group did not differ when compared to non-restraint group (R-C vs NRCF: 0.640±0.03vs0.745±0.02, P<0.05). At the behavioural level, open field studies showed that antidepressant treated animals were significantly less anxious than saline treated animals during post-restraint period (R-C vs R-FX: 0.092 ± 0.005vs0.1139 ± 0.004 CD/TD ratio, P <0.01).
Conclusion Our study suggests that short-term exposure to stress and antidepressants leads to long-term body weight gain accompanied with increased bone and spleen weights. These findings may implicate different pathophysiological mechanisms in stress and antidepressant related obesity when compared to obesity that is solely diet-induced.
Nothing to Disclose: SL, CAM, RL, GJP, PS, JL, MLW
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