Session: MON 355-388-Sex Hormone Receptor Action & Reaction
Poster Board MON-376
Nuclear Receptor Low Density Taqman Arrays were used to compare the gene expression profiles in a collection of primary cultured CAFs (n=9) and NAFs (n=7). StatMiner Analytical software was used to perform Non-Parametric (Wilcoxon) tests while geNorm selected the most stable endogenous controls used for normalisation. Thirty-three of 48 NRs were expressed in both cohorts, while 11 NRs were not detected in either. Three (DAX-1, ERR-b and ROR-b) were only detected in NAFs, whilst one (LRH-1) was unique to CAFs. Of the NRs expressed, four showed significant down-regulation in CAFs compared to NAFs: RAR-related orphan receptor-a (ROR-a) (1.95-fold); Thyroid hormone receptor-b (TR-b) (15.63-fold); Vitamin D receptor (VDR) (1.91-fold); and Peroxisome proliferator-activated receptor-g (PPAR-g) (3.48-fold).
Stromal expression of NRs may be an important component in the cross-talk within the tumor microenvironment. Given the importance of aromatase expression in the breast tumor stroma, our findings reveal a pattern of NR expression that fits with a sustained and increased local estrogen microenvironment. The elucidation of molecular mechanisms and functional consequences of the differing expression profiles of NRs in CAFs may provide a new avenue for the development of intratumoral-targeted therapies in breast cancer.
Nothing to Disclose: KCK, ALC, NE, KT, YM, HS, JV, GL, GEOM, CDC
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