Session: SAT 786-805-Diabetes & Obesity Therapeutics
Bench to Bedside
Poster Board SAT-790
Thus, the retardation of the action of these enzymes by suitable inhibitors may be one of the most effective approaches to control NIDDM. The aim of this work therefore, is to screen using cell free assay, for novel inhibitors for human starch degrading enzymes, salivary and pancreatic alpha-amylases, using natural resources and synthetic compounds. We prepared the total extracts of over two hundred herbs and plants collected from different locations in Egypt and in Qatar. Our study demonstrated that the total extract of four plants showed a significant inhibition of the both human alpha-amylase. The active compound from two plants has been isolated and its structure has been determined using many tools such as elemental analysis, IR, NMR, MS and 13C.
We also carried out cell fee assay inhibition study using over eight hundreds synthetic compounds collected from different chemistry groups in Egypt. Three synthetic compounds with a significant but variable degree of inhibition of human saliva alpha amylase have been identified. The structures of the three identified compounds were confirmed.
The novel inhibitors isolated in this study would form the base for screening for more inhibitors and the synthesis of the naturally occurring ones to confirm their structures. This will be followed by in vivo study to demonstrate the effectiveness of these compounds in lowering the glucose level in NIDDM.
Nothing to Disclose: SKG, FR, ME, FA, MA, VM
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