Isolation and Structural Determination of Naturally Occurring and Synthetic Inhibitors for Starch Degrading Enzymes: An effect approach for the control of diabetes mellitus and obesity

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SAT 786-805-Diabetes & Obesity Therapeutics
Bench to Bedside
Saturday, June 15, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SAT-790
Sayed Kamel Goda*1, Fatma Rashidy2, Mervat El sayed3, Fawzy Attaby3, Mohammed Al Sayrafi4 and Vidya Mohamed-Ali1
1Anti Doping Lab Qatar, Doha, Qatar, 2Cairo University, Cairo, Egypt, 3Cairo University, Egypt, 4Anti Doping Lab Qatar, Qatar
Non-insulin-dependent diabetes mellitus (NIDDM), which is one of the main adult diseases, is caused by the secretory decrease in insulin from pancreatic Langerhans cell and/ or peripheral cells become resistant to the action of insulin as in case of obesity. At present, the direct clinical therapy in NIDDM is to optimize or control the postprandial blood glucose (PBG) level. Polysaccharide degrading enzymes, such as alpha amylases catalyse the cleavage of starch to produce glucose which leads to the increase of its level in the blood.

Thus, the retardation of the action of these enzymes by suitable inhibitors may be one of the most effective approaches to control NIDDM. The aim of this work therefore, is to screen using cell free assay, for novel inhibitors for human starch degrading enzymes, salivary and pancreatic alpha-amylases, using natural resources and synthetic compounds. We prepared the total extracts of over two hundred herbs and plants collected from different locations in Egypt and in Qatar. Our study demonstrated that the total extract of four plants showed a significant inhibition of the both human alpha-amylase. The active compound from two plants has been isolated and its structure has been determined using many tools such as elemental analysis, IR, NMR, MS and 13C. 

We also carried out cell fee assay inhibition study using over eight hundreds synthetic compounds collected from different chemistry groups in Egypt. Three synthetic compounds with a significant but variable degree of inhibition of human saliva alpha amylase have been identified. The structures of the three identified compounds were confirmed.  

The novel inhibitors isolated in this study would form the base for screening for more inhibitors and the synthesis of the naturally occurring ones to confirm their structures. This will be followed by in vivo study to demonstrate the effectiveness of these compounds in lowering the glucose level in NIDDM.

Nothing to Disclose: SKG, FR, ME, FA, MA, VM

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Sources of Research Support: Anti-Doping Lab-Qatar, Doha, Qatar