Immune stress responses are altered by ovariectomy in female rats

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 515-547-Female Reproductive Endocrinology
Basic/Translational
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-529
Takeshi Iwasa*1, Toshiya Matsuzaki2, Riyo Kinouchi2, Ganbat Gereltsetseg2 and Minoru Irahara2
1Department of Obstetrics and Gynecology, The University of Tokushima Graduate School, Institute of Health Biosciences, Tokushima-Shi, Japan, 2Department of Obstetrics and Gynecology, The University of Tokushima Graduate School, Institute of Health Biosciences, Tokushima, Japan
[Objective]Both deficiency of gonadal steroids and obesity alter the stress response. Although ovariectomized (OVX) rodents, used as menopause models, show these two characteristics, their stress responses have not been evaluated. OVX-induced alterations of stress responses and the effects of gonadal steoids supplementation on these alterations were examined.

[Methods]Experiment 1:female rats were treated with OVX or sham operation (Sham) and stress responses were compared between these two groups. Stress was induced by LPS, and then anorectic response, hypothalamic IL-1beta and TNF-alpha mRNA, and serum leptin level were measured. Experiment 2:Either estradiol and progesterone (EP) or Vehicle (Veh) was acutely injected in OVX female rats, and then stress responses were compared as like as Experiment 1.

[Results]Experiment 1:Body weight in OVX rats was heavier than that in Sham rats. OVX rats exhibited an increased anorectic response, higher serum lepitn level and greater increase of hypothalamic IL-1beta mRNA expression compared with Sham rats. Experiment 2:There was no differences between OVX-EP rats and OVX-Veh rats in the anorectic response, serum lepitn level and hypothalamic IL-1beta mRNA.

[Conclusion]Stress responses to immune stress were increased in OVX rats. Indirect action of gonadal steroid deficiency, such as obesity, might be related in this alteration.

Nothing to Disclose: TI, TM, RK, GG, MI

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm