Proteomic Identification of Molecular Pathogenesis in Polycystic Ovary Syndrome Patients

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 515-547-Female Reproductive Endocrinology
Basic/Translational
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-534
Muhammad Akram*1 and Nabila Roohi2
1King Edward Medical University, Lahore, Pakistan, 2Department of Zoology (Endocrinology), University of the Punjab, Lahore, Pakistan
Background and aims: Incidence of PCOS in Pakistani women is considerably increasing, however, bulk of available clinical and aetiological data serves to provide only a framework for studying its genesis. Proteomic identification of serum signatures may be a valid tool for predicting and tracking the progression of comorbidities in women with PCOS.

Subjects and Methods: We assessed plasma proteome of 12 controls and 12 PCOS patients, aged 18-45 years. Following clinical examination, detailed menstrual and family history was recorded through a comprehensive questionnaire followed by ovarian ultrasound examination. The blood samples were processed for serum and proteins were analyzed by two dimensional gel electrophoresis using Protean IEF coupled with II XL Cell System. The resolved proteins were  identified by Swiss 2DPAGE database.

Results: By comparative proteome analysis, ten spots; three up-regulated and seven down-regulated were differentially expressed in PCOS patients in comparison to their healthy counterparts. The expressions of Immunoglobulin heavy chain, Haptoglobin beta chain, alpha-1 antitrypsin, alpha-1 acid glycoprotein, Apolipoprotein (Apo) A-I, Immunoglobulin light chain and Transthyretin were significantly suppressed (p<0.05), whereas, Serotransferrin, alpha-2-HS glycoprotein and Haptoglobin alpha chain indicated marked expressions (p<0.05) in PCOS compared to healthy women.

Conclusions: The altered expressions of ApoA-I, Haptoglobin and transthyretin predict that PCOS women are at a higher risk of future progression towards diabetes, insulin resistance and cardiovascular disorders, particularly, atherosclerosis. Further, the over-expressions of Serotransferrin and Haptoglobin alpha chain, whereas, declined expression of Haptoglobin beta chain demonstrate that variations in iron metabolism, oxidative stress and low grade chronic inflammation might be the culprits in molecular pathogenesis of PCOS in women.

Nothing to Disclose: MA, NR

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm