Session: MON 515-547-Female Reproductive Endocrinology
Poster Board MON-534
Subjects and Methods: We assessed plasma proteome of 12 controls and 12 PCOS patients, aged 18-45 years. Following clinical examination, detailed menstrual and family history was recorded through a comprehensive questionnaire followed by ovarian ultrasound examination. The blood samples were processed for serum and proteins were analyzed by two dimensional gel electrophoresis using Protean IEF coupled with II XL Cell System. The resolved proteins were identified by Swiss 2DPAGE database.
Results: By comparative proteome analysis, ten spots; three up-regulated and seven down-regulated were differentially expressed in PCOS patients in comparison to their healthy counterparts. The expressions of Immunoglobulin heavy chain, Haptoglobin beta chain, alpha-1 antitrypsin, alpha-1 acid glycoprotein, Apolipoprotein (Apo) A-I, Immunoglobulin light chain and Transthyretin were significantly suppressed (p<0.05), whereas, Serotransferrin, alpha-2-HS glycoprotein and Haptoglobin alpha chain indicated marked expressions (p<0.05) in PCOS compared to healthy women.
Conclusions: The altered expressions of ApoA-I, Haptoglobin and transthyretin predict that PCOS women are at a higher risk of future progression towards diabetes, insulin resistance and cardiovascular disorders, particularly, atherosclerosis. Further, the over-expressions of Serotransferrin and Haptoglobin alpha chain, whereas, declined expression of Haptoglobin beta chain demonstrate that variations in iron metabolism, oxidative stress and low grade chronic inflammation might be the culprits in molecular pathogenesis of PCOS in women.
Nothing to Disclose: MA, NR
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