Session: SUN 554-573-Ovarian & Uterine Function I
Poster Board SUN-573
Aims. To evaluate changes in the innervation and vascularization of the vaginal mucosae of post-menopausal women affected by diabetes and hypertension compared to healthy post-menopausal women.
Materials and Methods. Vaginal biopsies were obtained from healthy post-menopausal (Mc) (n=9) women and post-menopausal women with diagnosis of diabetes (Md) (n=9) or severe hypertension (Mh) (n=9), undergoing genital surgery. CD31 immunostaining was used to characterize the vaginal vascular bed while vaginal mucosa gene expression of the endothelial (eNOS) and neuronal (nNOS) nitroxyde synthase isoforms were determined by Real-Time PCR. Additionally vaginal protein expression of Protein Gene Product 9.5 (PGP9.5) and Tyrosine Hydroxylase (TH) were evaluated by Western Blot analysis.
Results CD31 immunostaining shows an increased vessel number in Mh and Md women (Mc:10,19±1,52; Md:10,85±0,51; Mh:14,11±1,04; P=<0.05) which are characterized by a reduced or absent vascular lumen as compared to Mc. Vaginal innervation as assessed by PGP9.5 staining was reduced in Md and Mh compared to Mc women (score: Mc:2,25±0,47; Md:1,70±0,17; Mh:1,01±0,12; P=<0.05). Mh women showed a slight increase in sympathetic innervation revealed by TH analysis (Mc:0,47±0,22; Md:0,44±0,26; Mh:0,78±0,20; P=n.s.). Both eNOS and nNOS gene expression were reduced in Md and Mh compared to Mc women reaching statistical significance only in the Md group (eNOS - Mc:6,27±2,58; Md:0,30±0,09; Mh:3,39±1,25 - nNOS - Mc:2,65±0,97; Md:0,55±1,18; Mh:1,28±0,36).
Conclusion. Our results suggest that vaginal mucosae from Md and Mh presents changes in innervation and vascularization compared to Mc. These changes may be at least partially responsible for the increased sexual dysfunctions in these women.
Nothing to Disclose: MB, GG, IM, ML, CMM
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