Session: SAT 389-413-Signaling and Transcriptional Control in Endocrine Systems
Poster Board SAT-404
After normalization and filtering, 43385 of the 61625 transcripts present on pangenomic arrays (Agilent PORCINET 60K) were found to be expressed in the adrenal glands. The multivariate analysis of expression profiles clearly distinguished the experimental groups, with the first axis (explaining 44% of variance) separating fetal ages and the second axis (explaining 30% of variance) separating genetic groups. 178 transcripts (FDR<5%) were significantly regulated with regard to the interaction between genotypes and gestational stages. The Ingenuity software (www.ingenuity.com) showed that among the differentially expressed genes (DEG) two functional networks were related to steroidogenesis and cytoskeleton structure and functions. The available promoter sequences of the DEG were inspected for transcription factors binding sites (TFBS) using most recent available matrices (JASPAR, TRANSFAC) and tools (cREMaG database (2)).
This approach provides novel insights into the molecular mechanisms controlling the process of gene transcription during the development of the adrenal gland and how they are related to physiology (HPA axis function, glycogen) and maturity phenotypes. The genes identified in the current study will also be candidates as biomarkers of pre-term maturity.
Nothing to Disclose: ET, LL, NI, YL, PM, YL, MS, PM
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