OR38-5 Effects of Prenatal Bisphenol-A and Postnatal Overfeeding on Cardiovascular Function in Sheep

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: OR38-Physiological Impacts of Endocrine Disrupting Chemicals
Monday, June 17, 2013: 11:15 AM-12:45 PM
Presentation Start Time: 12:15 PM
Room 256 (Moscone Center)
Puliyur S MohanKumar*1, Rajendran Thirumalai Doss2, Bari Oivier2, Anthony Pease2, Fernando Garcia2, Gregory D Fink2, Almudena Veiga-Lopez3, Vasantha Padmanabhan4 and Sheba M J MohanKumar5
1Michigan State Univ, Okemos, MI, 2Michigan State University, East Lansing, MI, 3Univ of Michigan Med Schl, Ann Arbor, MI, 4Univ of Michigan, Ann Arbor, MI, 5MICHIGAN STATE UNIV, East Lansing, MI
Bisphenol-A (BPA) is one of the widely used endocrine-disrupting chemicals (EDC). Prenatal exposure to BPA is known to impact birth weight. Low birth weight has been related to obesity and other metabolic disturbances during adulthood. The purpose of this study was to investigate the impact of prenatal BPA treatment and its interaction with postnatal overfeeding on cardiovascular function. Pregnant sheep were given daily subcutaneous injections of cottonseed oil (control) or BPA (0.5 mg/kg/day in cotton seed oil) from day 30 to 90 of gestation. Controls received vehicle. A subset of female offspring of these dams were overfed to increase bodyweight to ~30% over that of controls (OF group). The cardiovascular function of adult females was assessed using non-invasive echocardiography at 21 months of age. At the time of cardiovascular assessment, body weights of OF groups were higher compared to normal fed groups (p<0.05). Overfeeding increased systolic, diastolic and mean blood pressure (SBP, DBP and MBP) significantly in control animals (p<0.05). Prenatal BPA-treatment prevented this increase. There was a significant BPA treatment and diet interaction in SBP, DBP and MBP respectively (p<0.05).  A similar effect was observed in end systolic volume and left ventricular area during diastole (p<0.05). A significant diet effect was evident in interventricular septal thickness during systole (mm).  This parameter was significantly higher in OF controls (17.1±1.1) and OF-BPA (17.9±1.0) animals compared to normal fed control (14.6±1.1) and BPA (13.9±0.9) animals (p<0.05). There was a similar effect on left atrial diameter and left ventricular area during diastole, which was significantly higher in OF control animals compared to the rest of the groups (p<0.05). These data reveal that prenatal programming with BPA in combination with a postnatal modifier such as overfeeding can cause significant alterations in cardiovascular parameters in the offspring. Supported by R01ES016541.

Nothing to Disclose: PSM, RT, BO, AP, FG, GDF, AV, VP, SMJM

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm

Sources of Research Support: NIEHS R01ES016541