The ghrelin receptor antisense gene, GHSROS, in breast cancer progression

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SAT 292-325-Breast & Prostate Cancer
Basic
Saturday, June 15, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SAT-310
Patrick Thomas1, Eliza Whiteside1, Andrew Stacey1, Adrian C Herington2, Lisa Kerstin Chopin1 and Inge Seim*1
1QUT, Brisbane, Australia, 2Queensland Univ of Technology, Woolloongabba, Brisbane Q, Australia
Long non-coding RNAs have recently been recognised to play important roles in the regulation of key physiological and pathological processes, including differentiation, development and cancer.  A number of non-coding RNAs have been associated with cancer progression and are emerging as potential biomarkers. Here, we describe a novel, antisense long non-coding RNA, GHSROS (ghrelin secretagogue receptor opposite strand), which is encoded in the intronic region of the gene for the ghrelin receptor, the growth hormone secretagogue receptor (GHSR). Using strand-specific quantitative, real time RT-PCR, we demonstrate that GHSROS is expressed in a range of breast cancer cell lines and in clinical specimens from breast cancer patients. Transwell assays and xCELLigence real time cell analysis show that forced over-expression of the GHSROS gene stimulates increased cell migration in the immortalized, non-tumourigenic MCF10A cell line and in the metastatic MDA-MB231 breast cancer cell line. Expression analysis using DNA microarrays (and the MDA-MB231 cell line) demonstrates that GHSROS regulates the expression of a number of genes, including genes associated with breast cancer and cell migration.  As GHSROS is expressed in breast cancer and stimulates cell migration -an important process in cancer progression- it may provide a useful target for the development of novel therapies and diagnostics.

Nothing to Disclose: PT, EW, AS, ACH, LKC, IS

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm

Sources of Research Support: National Health and Medical Research Council, Australia; National Breast Cancer Foundation, Australia; The Cancer Council Queensland