Association of Sleep Disorders, Metabolic Derangements and Oxidative Stress

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: MON 596-630-Pediatric Endocrinology
Clinical
Monday, June 17, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board MON-624
Christopher John Dunne*1, Danna Tauber2, Karen Carvalho3, Shufang Wu4 and Francesco De Luca5
1St. Christopher's Hospital, Philadelphia, PA, 2St Christopher's Hospital, Philadelphia, PA, 3St Christopher's Hospital for Children, Philadelphia, PA, 4St. Christopher's Hospital for Children, Philadelphia, PA, 5St Christopher's Hospital for Children, Drexel University College of Medicine, Philadelphia, PA
Background:

We have previously demonstrated that, in children, sleep disorders and oxidative stress are independently associated with risk factors for cardio-vascular disease. Yet, little is known about the correlation between markers of disordered sleep and markers of oxidative stress in the pediatric population.

Methods:

We prospectively obtained urine samples from obese children with suspected sleep abnormalities undergoing nocturnal polysomnography. Samples were obtained in the evening prior to initiation (“PM” sample) and in the morning after the conclusion of the sleep study (“AM” sample).  Urinary 8-isoprostane and hydrogen peroxide (two established markers of oxidative stress) were measured using Elisa assays.  A fasting blood sample was obtained in the morning after the sleep study and the following parameters were measured: blood glucose and insulin, lipids, HgbA1C, and HOMA was calculated.

Results:

Thus far, we have analyzed the data relative to 18 children (9 males; mean age, 12.5 years). 7 were Hispanic (36.8%), 11 African-American (57.9%) and 1 Caucasian (5.3%).  The mean BMI z-score was 2.35 (standard deviation 0.533). Of these 18 children, 12 were found with an “abnormal” sleep study: 5 were diagnosed with primary snoring, 1 with upper airway resistance syndrome, and 6 with varying degrees of obstructive sleep apnea. 

Both the PM hydrogen peroxide and the PM 8-isoprostane levels were significantly correlated with their respective AM levels.   No significant correlation was found between either AM or PM levels of 8-isoprostane and the following sleep study parameters: total sleep time, sleep latency, % sleep time in REM, AHI, peak end tidal CO2.

In contrast, we found a significant inverse correlation between PM hydrogen peroxide, age, and fasting insulin, while AM hydrogen peroxide was correlated with BMI z-score and fasting glucose. Although not significant (p value 0.07), there was a trend towards an increased mean AM 8-isoprostane in those patients with polysomnographic evidence of sleep apnea (N=8) compared to those without sleep apnea (N=10) (mean levels 11318 pg/mL vs 5757 pg/mL).

Discussion:

These results are preliminary and should be interpreted with caution.  However, they suggest that oxidative stress is not consistently correlated with disordered sleep in children. Further analysis in a larger sample is warranted.

Nothing to Disclose: CJD, DT, KC, SW, FD

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm