Session: SAT 834-867-Islet Biology
Bench to Bedside
Poster Board SAT-855
Judith Molina, Alberto Fachado, Per-Olof Berggren, and Alejandro Caicedo
Acetylcholine is a neurotransmitter that is crucial for pancreatic beta cell function and survival. Less is known about how acetylcholine affects other endocrine cells within the pancreatic islet. Given that acetylcholine is released from alpha cells in the human islet, we hypothesized that cholinergic paracrine signaling affects the release of other hormones and paracrine signals within the islet. We investigated the expression of muscarinic receptors in human islet endocrine cells and found that beta cells express M3 and M5 receptors, alpha cells M4 receptors, and delta cells M1 receptors. Activation of these receptors increased insulin and somatostatin secretion from beta and delta cells, respectively. Activation of M4 receptors in alpha cells inhibited secretion of acetylcholine, indicating that acetylcholine regulates its own secretion via a negative feedback loop. Our results indicate that acetylcholine activates a different set of muscarinic receptors in each endocrine cell type, thus achieving cell-specific effects in the human islet. This allows for selective targeting in the context of therapeutic intervention in diabetes.
Nothing to Disclose: JTM, AFF, POB, AC
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