The Forkhead Transcription Factor Foxo1 is Required for Normal Somatotrope Function During Mouse Pituitary Development

Program: Abstracts - Orals, Featured Poster Presentations, and Posters
Session: SAT 164-196-Pituitary
Basic/Clinical
Saturday, June 15, 2013: 1:45 PM-3:45 PM
Expo Halls ABC (Moscone Center)

Poster Board SAT-189
Brock Kabat*, Mason Tippy, Deborah Osterholm Jung and Buffy Sue Ellsworth
SIU School of Medicine, Carbondale, IL
Congenital hypopituitarism is common, occurring in approximately one out of every 4,000 live births. Half of cases have unknown etiology. The long-term goal of our studies is to expand the molecular diagnoses for congenital hypopituitarism by identifying genes that contribute to this condition. The forkhead transcription factor FOXO1 is present in approximately half of somatotrope cells at embryonic day (e)18.5 and in adults.  This study sought to elucidate the role of FOXO1 in mouse pituitary development and function. Foxo1 was conditionally deleted from the pituitary using a Foxg1-Cre. Foxo1 conditional knockout mice exhibit significantly less growth hormone immunoreactivity and reduced expression of the growth hormone gene than wildtype mice at e18.5. These data suggest that Foxo1 is required for normal somatotrope function.  We are currently investigating whether other pituitary hormones are affected by loss of Foxo1 at e14.5 and e18.5. These studies identify Foxo1 as a candidate gene for congenital hypopituitarism.

Nothing to Disclose: BK, MT, DOJ, BSE

*Please take note of The Endocrine Society's News Embargo Policy at http://www.endo-society.org/endo2013/media.cfm

Sources of Research Support: This project was funded by a National Institute of Child Health and Human Development Grant R15HD063469 (B.S.E.) as well as a REACH Award (B.E.K.) and an ORDA Faculty Seed Grant (B.S.E.) from Southern Illinois University Carbondale, and a Central Research Committee Award (B.S.E.) from Southern Illinois University School of Medicine.